Evidence map›Paper›PMID 31035982›Full record

ArticleBMC biotechnology2019

Mutagenesis of FAD2 genes in peanut with CRISPR/Cas9 based gene editing.

Mei Yuan, Jun Zhu, Limin Gong, Liangqiong He, Crystal Lee, Suoyi Han, Charles Chen, Guohao He

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.

0numbers the graph read from it
0cells of the map it votes in
46citing papers in PubMed
4.2field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

46 citing papers in PubMed, 136 citations in OpenAlex.

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  10. Genome editing in grain legumes for food security.Frontiers in genome editing · 2025
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  11. Progress in genetic engineering and genome editing of peanuts: revealing the future of crop improvement.Physiology and molecular biology of plants : an international journal of functional plant biology · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Mei YuanTuskegee University, Tuskegee, AL, 36088, USA.
Jun ZhuTuskegee University, Tuskegee, AL, 36088, USA.
Limin GongTuskegee University, Tuskegee, AL, 36088, USA.
Liangqiong HeTuskegee University, Tuskegee, AL, 36088, USA.
Crystal LeeTuskegee University, Tuskegee, AL, 36088, USA.
Suoyi HanHenan Academy of Agricultural Sciences, Zhengzhou, 450002, China.
Charles ChenAuburn University, Auburn, AL, 36849, USA.
Guohao HeTuskegee University, Tuskegee, AL, 36088, USA. ghe@tuskegee.edu.
Tuskegee University · USAuburn University · USHenan Academy of Agricultural Sciences · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundIncreasing the content of oleic acid in peanut seeds is one of the major goals in peanut breeding due to consumer and industry benefits, such as anti-oxidation and long shelf-life. Homeologous ahFAD2A and ahFAD2B genes encode fatty acid desaturases, which are the key enzymes for converting oleic acid to linoleic acid that oxidizes readily. To date, all high oleic acid peanut varieties result from natural mutations occurred in both genes. A method to induce mutations in the genes of other elite cultivars could speed introgression of this valuable trait. The gene-editing approach utilizing CRISPR/Cas9 technology was employed to induce de novo mutations in the ahFAD2 genes using peanut protoplasts and hairy root cultures as models.

resultsThe hot spot of natural mutation in these genes was selected as the target region. Appropriate sgRNAs were designed and cloned into a CRISPR/Cas9 expression plasmid. As a result of CRISPR/Cas9 activity, three mutations were identified - G448A in ahFAD2A, and 441_442insA and G451T in ahFAD2B. The G448A and 441_442insA mutations are the same as those seen in existing high oleate varieties and the G451T is new mutation. Because natural mutations appear more often in the ahFAD2A gene than in the ahFAD2B gene in subspecies A. hypogaea var. hypogaea, the mutations induced in ahFAD2B by gene editing may be useful in developing high oleate lines with many genetic backgrounds after validation of oleic acid content in the transformed lines. The appearance of the G448A mutation in ahFAD2A is a further benefit for high oleic acid oil content.

conclusionsOverall, these results showed that mutations were, for the first time, induced by CRISPR-based gene editing approach in peanut. This research demonstrated the potential application of gene editing for mutagenesis in peanut and suggested that CRISPR/Cas9 technology may be useful in the peanut breeding programs.

Indexed as

CRISPR-Cas SystemsMutagenesisArachisBase SequenceFatty Acid DesaturasesGene EditingLinoleic AcidOleic AcidPlant BreedingPlant ProteinsPlants, Genetically ModifiedSeedsdelta-12 fatty acid desaturaseFatty Acid DesaturasesLinoleic AcidOleic AcidPlant ProteinsahFAD2 geneCRISPR/Cas9Gene-editingHigh oleic acid

Identifiers

PMID31035982
PMCPMC6489235
OpenAlexW2943724883

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.