Evidence map›Paper›PMID 31035923›Full record

ArticleThe journal of headache and pain2019

The effect of orofacial complete Freund's adjuvant treatment on the expression of migraine-related molecules.

Tamás Körtési, Bernadett Tuka, Aliz Nyári, László Vécsei, János Tajti

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The journal of headache and pain, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
2.6field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 29 citations in OpenAlex.

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  13. Protective Effects of PACAP in Peripheral Organs.Frontiers in endocrinology · 2020
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Tamás KörtésiDepartment of Neurology, Faculty of Medicine, Albert Szent-Györgyi Clinical Center University of Szeged, Semmelweis u. 6, Szeged, H-6725, Hungary.
Bernadett TukaMTA-SZTE Neuroscience Research Group, University of Szeged, Semmelweis u. 6, Szeged, H 6725, Hungary.
Aliz NyáriDepartment of Neurology, Faculty of Medicine, Albert Szent-Györgyi Clinical Center University of Szeged, Semmelweis u. 6, Szeged, H-6725, Hungary.
László VécseiMTA-SZTE Neuroscience Research Group, University of Szeged, Semmelweis u. 6, Szeged, H 6725, Hungary.
János TajtiDepartment of Neurology, Faculty of Medicine, Albert Szent-Györgyi Clinical Center University of Szeged, Semmelweis u. 6, Szeged, H-6725, Hungary. tajti.janos@med.u-szeged.hu.
University of Szeged · HU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMigraine is a neurovascular primary headache disorder, which causes significant socioeconomic problems worldwide. The pathomechanism of disease is enigmatic, but activation of the trigeminovascular system (TS) appears to be essential during the attack. Migraine research of recent years has focused on neuropeptides, such as calcitonin gene-related peptide (CGRP) and pituitary adenylate cyclase-activating polypeptide 1-38 (PACAP1-38) as potential pathogenic factors and possible therapeutic offensives. The goal of present study was to investigate the simultaneous expression of CGRP and precursor of PACAP1-38 (preproPACAP) in the central region of the TS in a time-dependent manner following TS activation in rats.

methodsThe right whisker pad of rats was injected with 50 μl Complete Freund's Adjuvant (CFA) or saline. A mechanical allodynia test was performed with von Frey filaments before and after treatment. Transcardial perfusion of the animals was initiated 24, 48, 72 and 120 h after injection, followed by the dissection of the nucleus trigeminus caudalis (TNC). After preparation, the samples were stored at - 80 °C until further use. The relative optical density of CGRP and preproPACAP was analyzed by Western blot. One-way ANOVA and Kruskal-Wallis followed by Tukey post hoc test were used to evaluate the data. Regression analysis was applied to explore the correlation between neuropeptides expression and hyperalgesia.

resultsOrofacial CFA injection resulted in significant CGRP and preproPACAP release in the TNC 24, 48, 72 and 120 h after the treatment. The level of neuropeptides reached its maximum at 72 h after CFA injection, corresponding to the peak of facial allodynia. Negative, linear correlation was detected between the expression level of neuropeptides and value of mechanonociceptive threshold.

conclusionThis is the first study which suggests that the expression of CGRP and preproPACAP simultaneously increases in the central region of activated TS and it influences the formation of mechanical hyperalgesia. Our results contribute to a better understanding of migraine pathogenesis and thereby to the development of more effective therapeutic approaches.

Indexed as

AnimalsCalcitonin Gene-Related PeptideFacial PainFreund's AdjuvantGene ExpressionHyperalgesiaMaleMigraine DisordersPeptide FragmentsPituitary Adenylate Cyclase-Activating PolypeptideRatsRats, Sprague-DawleyTrigeminal Caudal NucleusVibrissaeCalcitonin Gene-Related PeptideFreund's AdjuvantPeptide Fragmentspituitary adenylate cyclase-activating-peptide (1-38), pigPituitary Adenylate Cyclase-Activating PolypeptideCFACGRPMigrainepreproPACAPTrigeminovascular system

Identifiers

PMID31035923
PMCPMC6734445
OpenAlexW2947158236

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.