ArticleJournal of the American Chemical Society2019
Intercellular Instructed-Assembly Mimics Protein Dynamics To Induce Cell Spheroids.
Article in Journal of the American Chemical Society, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 86 citations in OpenAlex.
- Activatable NIR-II photoacoustic probes for cancer imaging and imaging-guided therapy.Bioactive materials · 2026Review
- Biomimetic peptide self-assembly: interfacing with biomacromolecules to regulate cellular signaling.Experimental & molecular medicine · 2026Review
- Timed photothermal therapy combining fluorescence-on chemotherapy maximizes tumor treatment.Bioactive materials · 2025Article
- Emerging self-assembled peptide hydrogels for enhanced wound healing.Nanomedicine (London, England) · 2025Review
- Self-Assembly of Noncanonical Peptides: A New Frontier in Cancer Therapeutics and Beyond.Macromolecular bioscience · 2025Review
- Intrinsically Disordered Peptide Nanofibers from a Structured Motif Within Proteins.Angewandte Chemie (International ed. in English) · 2025Article
- Nanofibers in Glioma Therapy: Advances, Applications, and Overcoming Challenges.International journal of nanomedicine · 2025Review
- Enzymatic self-assembly of short peptides for cell spheroid formation.Journal of materials chemistry. B · 2024Article
- Enzyme-Directed and Organelle-Specific Sphere-to-Fiber Nanotransformation Enhances Photodynamic Therapy in Cancer Cells.Small methods · 2024Article
- Phe-Phe-Based Macroscopic Supramolecular Hydrogel Construction Strategies and Biomedical Applications.Chem & bio engineering · 2024Review
- Engineering Tumor Stroma Morphogenesis Using Dynamic Cell-Matrix Spheroid Assembly.bioRxiv : the preprint server for biology · 2024Article
- Enzymatic control of intermolecular interactions for generating synthetic nanoarchitectures in cellular environment.Science and technology of advanced materials · 2024Review
- Therapeutic Supramolecular Polymers: Designs and Applications.Progress in polymer science · 2024Article
- Protocells Capable of Generating a Cytoskeleton-like Structure from intracellular Membrane-active Artificial Organelles.Advanced functional materials · 2023Article
- Enzyme-Instructed Intracellular Peptide Assemblies.Accounts of chemical research · 2023Article
- Peptide Assemblies for Cancer Therapy.ChemMedChem · 2023Review
- An enzyme-responsive and transformable PD-L1 blocking peptide-photosensitizer conjugate enables efficient photothermal immunotherapy for breast cancer.Bioactive materials · 2023Article
- Self-Assembly, Bioactivity, and Nanomaterials Applications of Peptide Conjugates with Bulky Aromatic Terminal Groups.ACS applied bio materials · 2023Review
- [Application of smart responsive materials in phosphopeptide and glycopeptide enrichment].Se pu = Chinese journal of chromatography · 2022Review
- Discovery of a phase-separating small molecule that selectively sequesters tubulin in cells.Chemical science · 2022Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Cell-mediated remodeling of extracellular matrix (ECM) plays important roles for cell functions, but it is challenging to develop synthetic materials for mimicking such a dynamic aspect of proteins in ECM. Here we show that intercellular morphological transition of peptide assemblies mimic the unfolding of fibronectin, thus enabling formation of spheroids from a monolayer of cells. Specifically, the phosphopeptide self-assembles to form nanoparticles, which turns into nanofibers upon partial dephosphorylation catalyzed by enzymes (e.g., phosphatases) at intercellular space. Occurring between HS-5 cells, such an enzyme-instructed self-assembly enables a sheet of the HS-5 cells to form cell spheroids. Structure-activity investigation reveals that proteolytic stability, dephosphorylation, and biotin conjugation of the peptides are indispensable for forming the cell spheroids. Further mechanism study indicates that the intercellular assemblies interact with multiple ECM components (e.g., laminin, collagens III and IV) to drive the formation of the cell spheroids. As the first example of intercellular instructed-assembly from homotypic precursors, this work illustrates a new approach that uses cell-responsive peptide assemblies to mimic protein dynamics for control cell behaviors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.