ATPase activity of human binding immunoglobulin protein (BiP) variants is enhanced by signal sequence and physiological concentrations of Mn

Sravanthi Bandla, Suraya Diaz, Heinz Peter Nasheuer, Una FitzGerald

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Article in FEBS open bio, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

0.1field-weighted citation impact, top 55% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

Sanguinarine and Doxorubicin Inhibit Heat Shock Protein HSPA5 and Trigger ER Stress in Breast Cancer.Cell biochemistry and biophysics · 2026
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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Sravanthi BandlaSchool of Natural Sciences, National University of Ireland Galway, Galway, Ireland.

Suraya DiazSchool of Natural Sciences, National University of Ireland Galway, Galway, Ireland.

Heinz Peter NasheuerSchool of Natural Sciences, National University of Ireland Galway, Galway, Ireland.

Una FitzGeraldSchool of Natural Sciences, National University of Ireland Galway, Galway, Ireland.

Ollscoil na Gaillimhe – University of Galway · IE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

B-cell immunoglobulin binding protein (BiP) is an essential endoplasmic reticulum (ER) chaperone normally found in the ER lumen. However, BiP also has other extracellular and intracellular functions. As it is unclear whether peripheral BiP has a signal and/or ER retention sequence, here we produced and biochemically characterised four variants of BiP. The variants differed depending on the presence or the absence of signal and ER retention peptides. Proteins were purified using nickel affinity chromatography, and variant size and quality were confirmed using SDS/PAGE gels. The thermal denaturing temperature of these proteins was found to be 46-47 °C. In addition, we characterised nucleotide binding properties in the absence and the presence of divalent cations. Interestingly, in the absence of cations, ADP has a higher binding affinity to BiP than ATP. The presence of divalent cations results in a decrease of the K

Indexed as

Adenosine TriphosphatasesAmino Acid SequenceCarrier ProteinsEndoplasmic ReticulumEndoplasmic Reticulum Chaperone BiPHeat-Shock ProteinsHomeostasisHumansImmunoglobulinsIon TransportLymphokinesMolecular ChaperonesProtein Sorting SignalsAdenosine TriphosphatasesCarrier ProteinsEndoplasmic Reticulum Chaperone BiPHeat-Shock Proteinsimmunoglobulin-binding factorsImmunoglobulinsLymphokinesMolecular ChaperonesProtein Sorting SignalsATPaseB-cell immunoglobulin binding proteinendoplasmic reticulum chaperoneendoplasmic reticulum retention sequence KDELGlucose-regulated protein 78 kDasignal sequence

Identifiers

PMID31033254

DOI10.1002/2211-5463.12645

PMCPMC6668376

OpenAlexW2942998599

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LicenceCC BY

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.