ArticleMolecular reproduction and development2019
The seminal acrosin-inhibitor ClTI1/SPINK2 is a fertility-associated marker in the chicken.
Article in Molecular reproduction and development, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 18 citations in OpenAlex.
- SPINK2 in hematopoiesis and cancer: Biology and clinical implications.Cell death discovery · 2026Review
- Experimental Evidence That Prenatal and Postnatal Developmental Stress Affects the Adult Seminal Fluid Proteome in a Precocial Bird.Molecular ecology · 2026Article
- Comparative Analysis of Proteomic Characteristics in Seminal Plasma Between Horses and Donkeys.Animals : an open access journal from MDPI · 2025Article
- Exploring how sucrose-colloid selection improves the fertilizing ability of chicken sperm after cryopreservation with glycerol.Poultry science · 2024Article
- A theoretical framework of immune cell phenotypic classification and discovery.Frontiers in immunology · 2023Article
- Three-dimensional structures of avian beta-microseminoproteins: insight from the chicken egg-specific beta-microseminoprotein 3 paralog.FEBS open bio · 2021Article
- Seminal Plasma: Relevant for Fertility?International journal of molecular sciences · 2021Review
- Elucidating the processes and pathways enriched in buffalo sperm proteome in regulating semen quality.Cell and tissue research · 2021Article
- Functional Aspects of Seminal Plasma in Bird Reproduction.International journal of molecular sciences · 2020Review
- Seminal Plasma Proteome as an Indicator of Sperm Dysfunction and Low Sperm Motility in Chickens.Molecular & cellular proteomics : MCP · 2020Article
- The seminal acrosin-inhibitor ClTI1/SPINK2 is a fertility-associated marker in the chicken.Molecular reproduction and development · 2019Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The seminal plasma is a very complex fluid, which surrounds sperm in semen. It contains numerous proteins including proteases and protease inhibitors that regulate proteolytic processes associated with protein activation and degradation. We previously identified a seminal protein, chicken liver trypsin inhibitor 1 (ClTI-1) over expressed in semen of roosters with high fertility, suggesting a role in male fertility. In the present study, we showed that ClTI-1 gene is actually SPINK2. Using normal healthy adult roosters, we showed that SPINK2 amount in seminal plasma was positively correlated with male fertility in chicken lines with highly contrasted genetic backgrounds (broiler and layer lines). Using affinity chromatography combined to mass spectrometry analysis and kinetic assays, we demonstrated for the first time that two chicken acrosin isoforms (acrosin and acrosin-like proteins) are the physiological serine protease targets of SPINK2 inhibitor. SPINK2 transcript was overexpressed all along the male tract, and the protein was present in the lumen as expected for secreted proteins. Altogether, these data emphasize the role of seminal SPINK2 Kazal-type inhibitor as an important actor of fertility in birds through its inhibitory action on acrosin isoforms proteins.
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