Evidence map›Paper›PMID 31032368›Full record

ArticleBioMed research international2019

Improvement of K562 Cell Line Transduction by FBS Mediated Attachment to the Cell Culture Plate.

Maryam Abbasalipour, Mohammad Ali Khosravi, Sirous Zeinali, Hossein Khanahmad, Morteza Karimipoor, Kayhan Azadmanesh

Open access · hybridAbstract read
In one paragraph

Article in BioMed research international, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
1.8field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 16 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Maryam AbbasalipourDepartment of Molecular Medicine, Biotechnology Research Center, Pasteur Institute of Iran, Tehran 1316943551, Iran.
Mohammad Ali KhosraviDepartment of Molecular Medicine, Biotechnology Research Center, Pasteur Institute of Iran, Tehran 1316943551, Iran.
Sirous ZeinaliDepartment of Molecular Medicine, Biotechnology Research Center, Pasteur Institute of Iran, Tehran 1316943551, Iran.
Hossein KhanahmadDepartment of Molecular Biology and Genetics, Isfahan University of Medical Science, Hezar-Jerib Ave., Isfahan 8174673461, Iran.
Morteza KarimipoorDepartment of Molecular Medicine, Biotechnology Research Center, Pasteur Institute of Iran, Tehran 1316943551, Iran.ORCID 0000-0002-2406-5963
Kayhan AzadmaneshDepartment of Virology, Pasteur Institute of Iran, Tehran 1316943551, Iran.ORCID 0000-0002-7165-9043
Pasteur Institute of Iran · IRUniversity of Isfahan · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Lentiviral vectors have been used for gene therapy in the clinical phase in recent years. These vectors provide a tool for gene insertion, deletion, or modification in organisms. The K562 human cell line has been used extensively in hematopoietic research. Despite its broad application, it is hard-to-transfection and transduction. So, this study presents a simple method to increase the transduction efficiency of K562 cells with a low multiplicity of infection (MOI) of the virus particle. For this purpose, 24-well plate was coated by 300

Indexed as

Genetic TherapyK562 CellsCell Culture TechniquesCulture MediaFlow CytometryGenetic VectorsHumansLentivirusTransduction, GeneticTransfectionCulture Media

Identifiers

PMID31032368
PMCPMC6457364
OpenAlexW2924184847

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.