Evidence map›Paper›PMID 31030840›Full record

ArticleVeterinary microbiology2019

Limited adaptation of chimeric H9N2 viruses containing internal genes from bat influenza viruses in chickens.

Chaochao Ren, Yinyin Chen, Min Zhang, Ting Zhang, Danqi Bao, Chenyang Lu, Ruixue Xue, Yanjie Zhang, Wenhua Liu, Hongjun Chen and 5 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Veterinary microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.8field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 4 institutions in 1 country.

Chaochao RenShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Yinyin ChenShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Min ZhangShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Ting ZhangShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Danqi BaoShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Chenyang LuShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Ruixue XueShandong Provincial Center for Animal Disease Control and Prevention, Jinan, Shandong, China.
Yanjie ZhangCollege of Animal Husbandry and Veterinary Medicine, Qingdao Agricultural University, Qingdao, China.
Wenhua LiuCollege of Animal Husbandry and Veterinary Medicine, Qingdao Agricultural University, Qingdao, China.
Hongjun ChenShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Qiaoyang TengShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Jianmei YangShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Xuesong LiShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Zejun LiShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China. Electronic address: lizejun@shvri.ac.cn.
Qinfang LiuShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China. Electronic address: liuqinfang@shvri.ac.cn.
Chinese Academy of Agricultural Sciences · CNShanghai Veterinary Research Institute · CNQingdao Agricultural University · CNShandong Center for Disease Control and Prevention · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Influenza virus-like sequences of H17N10 and H18N11 were identified in bats, despite there has been no live virus isolated. The genetic analysis indicated that they have distinct but relatively close evolutionary relationships to known influenza A viruses. However, the infectivity and adaptation of bat influenza viruses in avian species remain unclear. In this study, two modified bat influenza viruses cH9cN2/H17 and cH9cN2/H18 containing HA and NA coding regions replaced with those of H9N2 influenza A virus were generated in the background of the H17N10 or H18N11 viruses. These two modified viruses replicated less efficiently than wild type H9N2 virus in cultured chicken cells. The mini-genome assay showed that viral ribonucleoproteins (vRNPs) of H9N2 has significantly higher polymerase activity than that of bat influenza viruses in avian cells. In chicken study, compared with H9N2 virus, which replicated and transmitted efficiently in chickens, the cH9cN2/H17 and cH9cN2/H18 viruses only replicated in chicken tracheas with lower titers. Pathological examination showed that the H9N2 caused severer lesions in lung and trachea than the modified bat influenza viruses. Notably, the cH9cN2/H18 transmitted among chickens, but not cH9cN2/H17, and chicken IFN-β antagonism results showed that H18N11 NS1 protein inhibited chicken IFN-β response more efficiently than H17N10 NS1 protein in avian cells. Taken together, our data indicated that the internal genes of bat influenza viruses adapted poorly to chickens, while the internal genes of H18N11 seemed to adapt to chickens better than H17N10.

Indexed as

Adaptation, PhysiologicalAnimalsBase SequenceChickensChimeraChiropteraHemagglutinins, ViralInfluenza A Virus, H9N2 SubtypeInfluenza in BirdsNeuraminidaseOrthomyxoviridaePoultry DiseasesRibonucleoproteinsHemagglutinins, ViralNeuraminidaseRibonucleoproteinsBat influenza virusH17N10H18N11H9N2

Identifiers

PMID31030840
PMCPMC7117387
OpenAlexW2935853135

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.