Evidence map›Paper›PMID 31029957›Full record

ArticleSolid state nuclear magnetic resonance2019

A biradical-tagged phospholipid as a polarizing agent for solid-state MAS Dynamic Nuclear Polarization NMR of membrane proteins.

Daryl B Good, Maxim A Voinov, David Bolton, Meaghan E Ward, Ivan V Sergeyev, Marc Caporini, Peter Scheffer, Andy Lo, Melanie Rosay, Antonin Marek and 3 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Solid state nuclear magnetic resonance, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.1field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 14 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 2 countries.

Daryl B GoodDepartment of Physics and Biophysics Interdepartmental Group, University of Guelph, Guelph, Ontario, Canada.
Maxim A VoinovDepartment of Chemistry, North Carolina State University, Raleigh, NC, USA.
David BoltonDepartment of Physics and Biophysics Interdepartmental Group, University of Guelph, Guelph, Ontario, Canada.
Meaghan E WardDepartment of Physics and Biophysics Interdepartmental Group, University of Guelph, Guelph, Ontario, Canada.
Ivan V SergeyevBruker Biospin, Billerica, MA, USA.
Marc CaporiniBruker Biospin, Billerica, MA, USA.
Peter SchefferDepartment of Physics and Biophysics Interdepartmental Group, University of Guelph, Guelph, Ontario, Canada.
Andy LoDepartment of Physics and Biophysics Interdepartmental Group, University of Guelph, Guelph, Ontario, Canada.
Melanie RosayBruker Biospin, Billerica, MA, USA.
Antonin MarekDepartment of Chemistry, North Carolina State University, Raleigh, NC, USA.
Leonid S BrownDepartment of Physics and Biophysics Interdepartmental Group, University of Guelph, Guelph, Ontario, Canada.
Alex I SmirnovDepartment of Chemistry, North Carolina State University, Raleigh, NC, USA; Bruker Biospin, Billerica, MA, USA. Electronic address: aismirno@ncsu.edu.
Vlad LadizhanskyDepartment of Physics and Biophysics Interdepartmental Group, University of Guelph, Guelph, Ontario, Canada; Bruker Biospin, Billerica, MA, USA. Electronic address: vladizha@uoguelph.ca.
University of Guelph · CABruker (United States) · USNorth Carolina State University · US

Funding

Resonator Approach to Pulsed Dynamic Nuclear Polarization of Membrane ProteinsR01GM130821 · NIGMS · NORTH CAROLINA STATE UNIVERSITY RALEIGH · PI NEVZOROV, ALEXANDER A., SMIRNOV, ALEX I. · 2018 to 2021
$1.7M
Time-domain/ELDOR EPR SpectrometerS10RR023614 · NCRR · NORTH CAROLINA STATE UNIVERSITY RALEIGH · PI SMIRNOV, ALEX I. · 2009 to 2009
$500k
NCRR NIH HHS S10 RR023614NIGMS NIH HHS R01 GM130821
6 · The paper itself

Abstract

A novel Dynamic Nuclear Polarization (DNP) NMR polarizing agent ToSMTSL-PTE representing a phospholipid with a biradical TOTAPOL tethered to the polar head group has been synthesized, characterized, and employed to enhance solid-state Nuclear Magnetic Resonance (SSNMR) signal of a lipid-reconstituted integral membrane protein proteorhodopsin (PR). A matrix-free PR formulation for DNP improved the absolute sensitivity of NMR signal by a factor of ca. 4 compared to a conventional preparation with TOTAPOL dispersed in a glassy glycerol/water matrix. DNP enhancements measured at 400 MHz/263 GHz and 600 MHz/395 GHz showed a strong field dependence but remained moderate at both fields, and comparable to those obtained for PR covalently modified with ToSMTSL. Additional continuous wave (CW) X-band electron paramagnetic resonance (EPR) experiments with ToSMTSL-PTE in solutions and in lipid bilayers revealed that an unfavorable conformational change of the linker connecting mononitroxides could be one of the reasons for moderate DNP enhancements. Further, differential scanning calorimetry (DSC) and CW EPR experiments indicated an inhomogeneous distribution and/or a possibility of a partial aggregation of ToSMTSL-PTE in DMPC:DMPA bilayers when the concentration of the polarizing agent was increased to 20 mol% to maximize the DNP enhancement. Thus, conformational changes and an inhomogeneous distribution of the lipid-based biradicals in lipid bilayers emerged as important factors to consider for further development of this matrix-free approach for DNP of membrane proteins.

Indexed as

Magnetic Resonance SpectroscopyGlycerolLipid BilayersMembrane ProteinsPhospholipidsWaterGlycerolLipid BilayersMembrane ProteinsPhospholipidsWaterBilayerBiradicalDynamic nuclear polarizationElectron spin resonanceLipidMembrane proteinNuclear magnetic resonanceSignal enhancement

Identifiers

PMID31029957
PMCPMC6709687
OpenAlexW2937725836

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.