Evidence map›Paper›PMID 31029696›Full record

ArticleMolecular metabolism2019

Reduced insulin action in muscle of high fat diet rats over the diurnal cycle is not associated with defective insulin signaling.

Lewin Small, Amanda E Brandon, Benjamin L Parker, Vinita Deshpande, Azrah F Samsudeen, Greg M Kowalski, Jane Reznick, Donna L Wilks, Elaine Preston, Clinton R Bruce and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular metabolism, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.8field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 16 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 4 institutions in 1 country.

Lewin SmallDiabetes and Metabolism Division, The Garvan Institute of Medical Research, Sydney, NSW, Australia.
Amanda E BrandonDiabetes and Metabolism Division, The Garvan Institute of Medical Research, Sydney, NSW, Australia; The University of Sydney, School of Medical Science, Charles Perkins Centre D17, Sydney, NSW, Australia.
Benjamin L ParkerThe University of Sydney, School of Life and Environmental Science, Charles Perkins Centre D17, Sydney, NSW, Australia.
Vinita DeshpandeThe University of Sydney, School of Life and Environmental Science, Charles Perkins Centre D17, Sydney, NSW, Australia.
Azrah F SamsudeenDepartment of Pharmacology, School of Medical Science, University of New South Wales, Sydney, NSW, Australia.
Greg M KowalskiDeakin University, School of Exercise and Nutrition Sciences, Faculty of Health, Institute for Physical Activity and Nutrition, Geelong, Australia.
Jane ReznickDiabetes and Metabolism Division, The Garvan Institute of Medical Research, Sydney, NSW, Australia.
Donna L WilksDiabetes and Metabolism Division, The Garvan Institute of Medical Research, Sydney, NSW, Australia.
Elaine PrestonDiabetes and Metabolism Division, The Garvan Institute of Medical Research, Sydney, NSW, Australia.
Clinton R BruceDeakin University, School of Exercise and Nutrition Sciences, Faculty of Health, Institute for Physical Activity and Nutrition, Geelong, Australia.
David E JamesThe University of Sydney, School of Life and Environmental Science, Charles Perkins Centre D17, Sydney, NSW, Australia.
Nigel TurnerDepartment of Pharmacology, School of Medical Science, University of New South Wales, Sydney, NSW, Australia.
Gregory J CooneyDiabetes and Metabolism Division, The Garvan Institute of Medical Research, Sydney, NSW, Australia; The University of Sydney, School of Medical Science, Charles Perkins Centre D17, Sydney, NSW, Australia. Electronic address: gregory.cooney@sydney.edu.au.
Garvan Institute of Medical Research · AUUniversity of Sydney · AUDeakin University · AUUNSW Sydney · AU

Funding

Regional Pilot And Feasibility Study Grants ProgramP30DK020572 · NIDDK · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Mehboob A Hussain · 2013 to 2026
$24.3M
NIDDK NIH HHS P30 DK020572
6 · The paper itself

Abstract

objectiveEnergy metabolism and insulin action follow a diurnal rhythm. It is therefore important that investigations into dysregulation of these pathways are relevant to the physiology of this diurnal rhythm.

methodsWe examined glucose uptake, markers of insulin action, and the phosphorylation of insulin signaling intermediates in muscle of chow and high fat, high sucrose (HFHS) diet-fed rats over the normal diurnal cycle.

resultsHFHS animals displayed hyperinsulinemia but had reduced systemic glucose disposal and lower muscle glucose uptake during the feeding period. Analysis of gene expression, enzyme activity, protein abundance and phosphorylation revealed a clear diurnal regulation of substrate oxidation pathways with no difference in Akt signaling in muscle. Transfection of a constitutively active Akt2 into the muscle of HFHS rats did not rescue diet-induced reductions in insulin-stimulated glucose uptake.

conclusionsThese studies suggest that reduced glucose uptake in muscle during the diurnal cycle induced by short-term HFHS-feeding is not the result of reduced insulin signaling.

Indexed as

AnimalsBlood GlucoseCircadian RhythmDiet, High-FatDisease Models, AnimalEnergy MetabolismGene ExpressionInsulinInsulin ResistanceMaleMuscle, SkeletalPhosphorylationProto-Oncogene Proteins c-aktRatsRats, WistarRNA, MessengerAkt2 protein, ratBlood GlucoseInsulinProto-Oncogene Proteins c-aktRNA, MessengerDiurnal rhythmsGlucose uptakeInsulin actionInsulin signalingPhosphoproteomicsSkeletal muscle

Identifiers

PMID31029696
PMCPMC6600078
OpenAlexW2936855091

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.