ArticleApplied and environmental microbiology2019
Viable and Total Bacterial Populations Undergo Equipment- and Time-Dependent Shifts during Milk Processing.
Article in Applied and environmental microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 48 citations in OpenAlex.
- The Impact of Cold Storage and Seasonality on Raw Cow Milk Microbiota, Assessed by Conventional and Viability PCR.Animals : an open access journal from MDPI · 2026Article
- Variations in cow milk and teat skin microbiota across the lactation cycle with intramammary cephalosporin use at dry-off.Applied and environmental microbiology · 2026Article
- Article
- Step-by-Step Metagenomics for Food Microbiome Analysis: A Detailed Review.Foods (Basel, Switzerland) · 2024Review
- Murine cartilage microbial DNA deposition occurs rapidly following the introduction of a gut microbiome and changes with obesity, aging, and knee osteoarthritis.GeroScience · 2024Article
- Article
- Analytical methods for assessing antimicrobial activity of nanomaterials in complex media: advances, challenges, and perspectives.Journal of nanobiotechnology · 2023Review
- The Use of Ozone Technology: An Eco-Friendly Method for the Sanitization of the Dairy Supply Chain.Foods (Basel, Switzerland) · 2023Review
- Extending the Shelf Life of Raw Milk and Pasteurized Milk with Plantaricin FB-2.Foods (Basel, Switzerland) · 2023Article
- Development of sequencing-based methodologies to distinguish viable from non-viable cells in a bovine milk matrix: A pilot study.Frontiers in microbiology · 2022Article
- A Flagella Hook Coding GeneFrontiers in microbiology · 2022Article
- Improved assessments of bulk milk microbiota composition via sample preparation and DNA extraction methods.PloS one · 2022Article
- Seasonality and Geography Have a Greater Influence than the Use of Chlorine-Based Cleaning Agents on the Microbiota of Bulk Tank Raw Milk.Applied and environmental microbiology · 2021Article
- Microbiota Assessments for the Identification and Confirmation of Slit Defect-Causing Bacteria in Milk and Cheddar Cheese.mSystems · 2021Article
- The Cheese Production Facility Microbiome Exhibits Temporal and Spatial Variability.Frontiers in microbiology · 2021Article
- Complementary Use of Cultivation and High-Throughput Amplicon Sequencing Reveals High Biodiversity Within Raw Milk Microbiota.Frontiers in microbiology · 2020Article
- Gaps in the assortment of rapid assays for microorganisms of interest to the dairy industry.Advances in applied microbiology · 2020Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We set out to identify the viable and total bacterial content in milk as it passes through a large-scale, dairy product manufacturing plant for pasteurization, concentration, separation, blending, and storage prior to cheese manufacture. A total of 142 milk samples were collected from up to 10 pieces of equipment for a period spanning 21 h on two collection dates in the spring and late summer of 2014. Bacterial composition in the milk was determined by 16S rRNA marker gene, high-throughput DNA sequencing. Milk samples from the late summer were paired such that half were treated with propidium monoazide (PMA) to enrich for viable cells prior to quantification by PCR and identification by DNA sequence analysis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.