Evidence map›Paper›PMID 31027908›Full record

ArticleDental materials : official publication of the Academy of Dental Materials2019

Enhancing cell seeding and osteogenesis of MSCs on 3D printed scaffolds through injectable BMP2 immobilized ECM-Mimetic gel.

Farahnaz Fahimipour, Erfan Dashtimoghadam, Mohammad Mahdi Hasani-Sadrabadi, Jessica Vargas, Daryoosh Vashaee, Douglas C Lobner, Tahereh S Jafarzadeh Kashi, Behnam Ghasemzadeh, Lobat Tayebi

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In one paragraph

Article in Dental materials : official publication of the Academy of Dental Materials, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
33citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

33 citing papers in PubMed, 1 synthesis or guideline pooled it.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Farahnaz FahimipourMarquette University School of Dentistry, Milwaukee, WI 53233, USA; Dental Biomaterials Department, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
Erfan DashtimoghadamMarquette University School of Dentistry, Milwaukee, WI 53233, USA.
Mohammad Mahdi Hasani-SadrabadiParker H. Petit Institute for Bioengineering and Bioscience, G.W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA; Weintraub Center for Reconstructive Biotechnology, Division of Advanced Prothodontics, School of Dentistry, University of California, Los Angeles, CA 90095, USA.
Jessica VargasMarquette University School of Dentistry, Milwaukee, WI 53233, USA.
Daryoosh VashaeeElectrical and Computer Engineering Department, North Carolina State University, Raleigh, NC 27606, USA.
Douglas C LobnerDepartment of Biomedical Sciences, Marquette University, Milwaukee, WI 53233, USA.
Tahereh S Jafarzadeh KashiDental Biomaterials Department, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
Behnam GhasemzadehDepartment of Biomedical Sciences, Integrative Neuroscience Research Center, Marquette University, Milwaukee, WI 53201, USA.
Lobat TayebiMarquette University School of Dentistry, Milwaukee, WI 53233, USA. Electronic address: lobat.tayebi@marquette.edu.

Funding

Supplement: Development of an Integrated 3D Human Osteo-Mucosal ModelR15DE027533 · NIDCR · MARQUETTE UNIVERSITY · PI TAYEBI, LOBAT · 2018 to 2021
$816k
NIDCR NIH HHS R15 DE027533
6 · The paper itself

Abstract

objectiveDesign of bioactive scaffolds with osteogenic capacity is a central challenge in cell-based patient-specific bone tissue engineering. Efficient and spatially uniform seeding of (stem) cells onto such constructs is vital to attain functional tissues. Herein we developed heparin functionalized collagen gels supported by 3D printed bioceramic scaffolds, as bone extracellular matrix (ECM)-mimetic matrices. These matrices were designed to enhance cell seeding efficiency of mesenchymal stem cells (MSCs) as well as improve their osteogenic differentiation through immobilized bone morphogenic protein 2 (BMP2) to be used for personalized bone regeneration.

methodsA 3D gel based on heparin-conjugated collagen matrix capable of immobilizing recombinant human bone morphogenic protein 2 (BMP2) was synthesized. Isolated dental pulp Mesenchymal stem cells (MSCs) were then encapsulated into the bone ECM microenvironment to efficiently and uniformly seed a bioactive ceramic-based scaffold fabricated using additive manufacturing technique. The designed 3D cell-laden constructs were comprehensively investigated trough in vitro assays and in vivo study.

resultsIn-depth rheological characterizations of heparin-conjugated collagen gel revealed that elasticity of the matrix is significantly improved compared with freely incorporated heparin. Investigation of the MSCs laden collagen-heparin hydrogels revealed their capability to provide spatiotemporal bioavailability of BMP2 while suppressing the matrix contraction over time. The in vivo histology and real-time polymerase chain reaction (qPCR) analysis showed that the designed construct supported the osteogenic differentiation of MSCs and induced the ectopic bone formation in rat model. SIGNIFICANCE: The presented hybrid constructs combine bone ECM chemical cues with mechanical function providing an ideal 3D microenvironment for patient-specific bone tissue engineering and cell therapy applications. The implemented methodology in design of ECM-mimetic 3D matrix capable of immobilizing BMP2 to improve seeding efficiency of customized scaffolds can be exploited for other bioactive molecules.

Indexed as

Mesenchymal Stem CellsOsteogenesisAnimalsBone Morphogenetic Protein 2Cell DifferentiationExtracellular MatrixHumansPrinting, Three-DimensionalRatsTissue EngineeringTissue ScaffoldsBMP2 protein, humanBone Morphogenetic Protein 23D printingBone morphogenic protein 2Bone tissue engineeringECM-mimeticInjectable thermogelin situ seeding

Identifiers

PMID31027908
PMCPMC7193177

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.