ArticleProteomes2019
Isolation and Characterization of Small Extracellular Vesicles from Porcine Blood Plasma, Cerebrospinal Fluid, and Seminal Plasma.
Article in Proteomes, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
39 citing papers in PubMed, 1 synthesis or guideline pooled it, 63 citations in OpenAlex.
- Seminal Extracellular Vesicles and Their Involvement in Male (In)Fertility: A Systematic Review.International journal of molecular sciences · 2023Pooled it
- Seminal Extracellular Vesicles as Molecular Modulators of Male Reproductive Function.Reproduction in domestic animals = Zuchthygiene · 2026Review
- Placental Small Extracellular Vesicles Undetected in Cerebrospinal Fluid of Preeclamptic and Eclamptic Women.Biomolecules · 2026Article
- Molecular Profiling of Extracellular Vesicles Isolated from Boar Reproductive Fluids.Journal of extracellular vesicles · 2026Article
- Labeling, isolation and characterization of cell-type-specific exosomes derived from mouse skin tissue.Nature protocols · 2026Review
- Proteomic profiling of porcine seminal extracellular vesicles reveals potential in vivo fertility biomarkers.Andrology · 2026Article
- Surface Problem: Unveiling Extracellular Vesicles Surface Markers and Their Implications for Central Nervous System Diseases' Diagnosis and Treatment.Molecular neurobiology · 2025Review
- MiRNA-29b accelerates the PDGF in exosomes and stimulates hepatic stellate cells to promote liver fibrosis in biliary atresia.European journal of medical research · 2025Article
- Article
- Protective role of extracellular vesicles against oxidative DNA damage.Biological research · 2025Article
- Exosomal circRNAs: The Key Role and Potential Therapeutic Target in Gastric Cancer.Current cancer drug targets · 2025Review
- Protocol for the isolation and characterization of porcine brain region-associated extracellular particles.PloS one · 2025Article
- The extracellular vesicles of boar seminal plasma contain oxytocin at levels associated with fertility.Extracellular vesicles and circulating nucleic acids · 2025Article
- A Size-Exclusion Chromatography-Based Procedure for Isolating Extracellular Vesicle Subsets from Porcine Seminal Plasma.Methods in molecular biology (Clifton, N.J.) · 2025Article
- RNA profiles differ between small and large extracellular vesicle subsets isolated from porcine seminal plasma.BMC genomics · 2024Article
- Challenges of MS-based small extracellular vesicles proteomics.Journal of extracellular vesicles · 2024Review
- Updating Research on Extracellular Vesicles of the Male Reproductive Tract in Farm Animals: A Systematic Review.Animals : an open access journal from MDPI · 2024Review
- Phosphorylated tau in cerebrospinal fluid-derived extracellular vesicles in Alzheimer's disease: a pilot study.Scientific reports · 2024Article
- Cryogenic electron microscopy reveals morphologically distinct subtypes of extracellular vesicles among porcine ejaculate fractions.Scientific reports · 2024Article
- Possible Roles of Extracellular Vesicles in the Pathogenesis and Interventions of Immune-Mediated Central Demyelinating Diseases.Experimental neurobiology · 2024Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Extracellular vesicles (EVs) are a highly attractive subject of biomedical research as possible carriers of nucleic acid and protein biomarkers. EVs released to body fluids enable indirect access to inner organs by so-called "liquid biopsies". Obtaining a high-quality EV sample with minimum contaminants is crucial for proteomic analyses using LC-MS/MS or other techniques. However, the EV content in various body fluids largely differs, which may hamper subsequent analyses. Here, we present a comparison of extracellular vesicle yields from blood plasma, cerebrospinal fluid, and seminal plasma using an experimental pig model. Pigs are widely used in biomedical research as large animal models with anatomy and physiology close to those of humans and enable studies (e.g., of the nervous system) that are unfeasible in humans. EVs were isolated from body fluids by differential centrifugation followed by ultracentrifugation. EVs were characterized according to protein yields and to the quality of the isolated vesicles (e.g., size distribution, morphology, positivity for exosome markers). In our experimental setting, substantial differences in EV amounts were identified among body fluids, with the seminal plasma being the richest EV source. The yields of pellet proteins from ultracentrifugation of 1 mL of porcine body fluids may help to estimate body fluid input volumes to obtain sufficient samples for subsequent proteomic analyses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.