ArticleiScience2019
Genetically Encoded Fluorescent Indicator GRAPHIC Delineates Intercellular Connections.
Article in iScience, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Fluorescent indicators for visualizing dynamic contact between cells and between processes originating from a single cell.Cell reports methods · 2026Article
- Droplet-based functional CRISPR screening of cell-cell interactions by SPEAC-seq.Nature protocols · 2025Review
- Enabling neighbour labelling: using synthetic biology to explore how cells influence their neighbours.Development (Cambridge, England) · 2024Review
- Secretory GFP reconstitution labeling of neighboring cells interrogates cell-cell interactions in metastatic niches.Nature communications · 2023Article
- Fluorescent sensors for imaging of interstitial calcium.Nature communications · 2023Article
- Visualization ofiScience · 2023Article
- The cytoplasmic localization of ADNP through 14-3-3 promotes sex-dependent neuronal morphogenesis, cortical connectivity, and calcium signaling.Molecular psychiatry · 2023Article
- Generalized Bioluminescent Platform To Observe and Track Cellular Interactions.Bioconjugate chemistry · 2022Article
- Development of intensiometric indicators for visualizing N-cadherin interaction across cells.Communications biology · 2022Article
- Molecular cell identities in the mediodorsal thalamus of infant mice and marmoset.The Journal of comparative neurology · 2022Article
- Tripartite synaptomics: Cell-surface proximity labeling in vivo.Neuroscience research · 2021Review
- Development of FRET-based indicators for visualizing homophilic trans interaction of a clustered protocadherin.Scientific reports · 2021Article
- Caged Cumate Enables Proximity-Dependent Control Over Gene Expression.Chembiochem : a European journal of chemical biology · 2021Article
- Article
- Protein Proximity Observed Using Fluorogen Activating Protein and Dye Activated by Proximal Anchoring (FAP-DAPA) System.ACS chemical biology · 2020Article
- Illuminating dendritic function with computational models.Nature reviews. Neuroscience · 2020Review
- Article
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Intercellular contacts are essential for precise organ morphogenesis, function, and maintenance; however, spatiotemporal information of cell-cell contacts or adhesions remains elusive in many systems. We developed a genetically encoded fluorescent indicator for intercellular contacts with optimized intercellular GFP reconstitution using glycosylphosphatidylinositol (GPI) anchor, GRAPHIC (GPI anchored reconstitution-activated proteins highlight intercellular connections), which can be used for an expanded number of cell types. We observed a robust GFP signal specifically at the interface between cultured cells, without disrupting natural cell contact. Application of GRAPHIC to the fish retina specifically delineated cone-bipolar connection sites. Moreover, we showed that GRAPHIC can be used in the mouse central nervous system to delineate synaptic sites in the thalamocortical circuit. Finally, we generated GRAPHIC color variants, enabling detection of multiple convergent contacts simultaneously in cell culture system. We demonstrated that GRAPHIC has high sensitivity and versatility, which will facilitate the analysis of the complex multicellular connections without previous limitations.
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Registered trials
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