Evidence map›Paper›PMID 31026495›Full record

ArticleCellular signalling2019

A fission yeast platform for heterologous expression of mammalian adenylyl cyclases and high throughput screening.

Rachel A Getz, Grace Kwak, Stacie Cornell, Samuel Mbugua, Jeremy Eberhard, Sheng Xiang Huang, Zainab Abbasi, Ana Santos de Medeiros, Rony Thomas, Brett Bukowski and 3 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cellular signalling, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.4field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 8 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Review
  5. cAMP export by the fission yeastmicroPublication biology · 2021
    Article
  6. Review
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 2 institutions in 1 country.

Rachel A GetzBiology Department, Boston College, 140 Commonwealth Ave., Chestnut Hill, MA 02467, USA.
Grace KwakBiology Department, Boston College, 140 Commonwealth Ave., Chestnut Hill, MA 02467, USA.
Stacie CornellBiology Department, Boston College, 140 Commonwealth Ave., Chestnut Hill, MA 02467, USA.
Samuel MbuguaBiology Department, Boston College, 140 Commonwealth Ave., Chestnut Hill, MA 02467, USA. Electronic address: mbugua@bc.edu.
Jeremy EberhardBiology Department, Boston College, 140 Commonwealth Ave., Chestnut Hill, MA 02467, USA. Electronic address: eberharj@bc.edu.
Sheng Xiang HuangBiology Department, Boston College, 140 Commonwealth Ave., Chestnut Hill, MA 02467, USA. Electronic address: huangsi@bc.edu.
Zainab AbbasiBiology Department, Boston College, 140 Commonwealth Ave., Chestnut Hill, MA 02467, USA. Electronic address: abbasiz@bc.edu.
Ana Santos de MedeirosBiology Department, Boston College, 140 Commonwealth Ave., Chestnut Hill, MA 02467, USA.
Rony ThomasBiology Department, Boston College, 140 Commonwealth Ave., Chestnut Hill, MA 02467, USA. Electronic address: thomasry@bc.edu.
Brett BukowskiBiology Department, Boston College, 140 Commonwealth Ave., Chestnut Hill, MA 02467, USA. Electronic address: bukowskb@bc.edu.
Patricia K DranchakNational Center for Advancing Translational Sciences, National Institutes of Health, Rockville, MD 20850, USA. Electronic address: patricia.dranchak@nih.gov.
James IngleseNational Center for Advancing Translational Sciences, National Institutes of Health, Rockville, MD 20850, USA. Electronic address: jinglese@mail.nih.gov.
Charles S HoffmanBiology Department, Boston College, 140 Commonwealth Ave., Chestnut Hill, MA 02467, USA. Electronic address: hoffmacs@bc.edu.
Boston College · USNational Institutes of Health · US

Funding

Target-based Assays and Screening Strategies for Chemical Probe and Therapeutic Lead DiscoveryZIATR000053 · NCATS · NATIONAL CENTER FOR ADVANCING TRANSLATIONAL SCIENCES · PI INGLESE, JAMES · 2015 to 2025
$4.5M
Intramural NIH HHS Z99 TR999999Intramural NIH HHS ZIA TR000053
6 · The paper itself

Abstract

The fission yeast Schizosaccharomyces pombe uses a cAMP signaling pathway to link glucose-sensing to Protein Kinase A activity in order to regulate cell growth, sexual development, gluconeogenesis, and exit from stationary phase. We previously used a PKA-repressed fbp1-ura4 reporter to conduct high throughput screens (HTSs) for inhibitors of heterologously-expressed mammalian cyclic nucleotide phosphodiesterases (PDEs). Here, we describe the successful expression of all ten mammalian adenylyl cyclase (AC) genes, along with the human GNAS Gα

Indexed as

Adenylyl CyclasesAnimalsCloning, MolecularHigh-Throughput Screening AssaysHumansSchizosaccharomycesAdenylyl CyclasesAdenylyl cyclaseDiphenyleneiodonium chlorideHigh-throughput screenSchizosaccharomyces pombe

Identifiers

PMID31026495
PMCPMC6662940
OpenAlexW2942153612

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.