Evidence map›Paper›PMID 31025141›Full record

ArticleCardiovascular drugs and therapy2019

Plasma from Volunteers Breathing Helium Reduces Hypoxia-Induced Cell Damage in Human Endothelial Cells-Mechanisms of Remote Protection Against Hypoxia by Helium.

Kirsten F Smit, Gezina T M L Oei, Moritz Konkel, Quinten J J Augustijn, Markus W Hollmann, Benedikt Preckel, Hemal H Patel, Nina C Weber

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cardiovascular drugs and therapy, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.9field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 12 citations in OpenAlex.

  1. Trial
  2. Article
  3. Review
  4. Article
  5. Review
  6. Review
  7. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Kirsten F SmitDepartment of Anesthesiology, Laboratory of Experimental Intensive Care and Anesthesiology (L.E.I.C.A), AUMC, Academic Medical Centre (AMC), University of Amsterdam, Meibergdreef 9, 1100 DD, Amsterdam, The Netherlands.
Gezina T M L OeiDepartment of Anesthesiology, Laboratory of Experimental Intensive Care and Anesthesiology (L.E.I.C.A), AUMC, Academic Medical Centre (AMC), University of Amsterdam, Meibergdreef 9, 1100 DD, Amsterdam, The Netherlands.
Moritz KonkelDepartment of Anesthesiology, Laboratory of Experimental Intensive Care and Anesthesiology (L.E.I.C.A), AUMC, Academic Medical Centre (AMC), University of Amsterdam, Meibergdreef 9, 1100 DD, Amsterdam, The Netherlands.
Quinten J J AugustijnDepartment of Anesthesiology, Laboratory of Experimental Intensive Care and Anesthesiology (L.E.I.C.A), AUMC, Academic Medical Centre (AMC), University of Amsterdam, Meibergdreef 9, 1100 DD, Amsterdam, The Netherlands.
Markus W HollmannDepartment of Anesthesiology, Laboratory of Experimental Intensive Care and Anesthesiology (L.E.I.C.A), AUMC, Academic Medical Centre (AMC), University of Amsterdam, Meibergdreef 9, 1100 DD, Amsterdam, The Netherlands.
Benedikt PreckelDepartment of Anesthesiology, Laboratory of Experimental Intensive Care and Anesthesiology (L.E.I.C.A), AUMC, Academic Medical Centre (AMC), University of Amsterdam, Meibergdreef 9, 1100 DD, Amsterdam, The Netherlands. B.Preckel@amc.uva.nl.
Hemal H PatelDepartment of Anesthesiology, University of California, San Diego and VA San Diego Healthcare System, San Diego, CA, USA.
Nina C WeberDepartment of Anesthesiology, Laboratory of Experimental Intensive Care and Anesthesiology (L.E.I.C.A), AUMC, Academic Medical Centre (AMC), University of Amsterdam, Meibergdreef 9, 1100 DD, Amsterdam, The Netherlands.
University of Amsterdam · NLVA San Diego Healthcare System · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeRemote ischemic preconditioning protects peripheral organs against prolonged ischemia/reperfusion injury via circulating protective factors. Preconditioning with helium protected healthy volunteers against postischemic endothelial dysfunction. We investigated whether plasma from helium-treated volunteers can protect human umbilical vein endothelial cells (HUVECs) against hypoxia in vitro through release of circulating of factors.

methodsHealthy male volunteers inhaled heliox (79% helium, 21% oxygen) or air for 30 min. Plasma was collected at baseline, directly after inhalation, 6 h and 24 h after start of the experiment. HUVECs were incubated with either 5% or 10% of the plasma for 1 or 2 h and subjected to enzymatically induced hypoxia. Cell damage was measured by LDH content. Furthermore, caveolin 1 (Cav-1), hypoxia-inducible factor (HIF1α), extracellular signal-regulated kinase (ERK)1/2, signal transducer and activator of transcription (STAT3) and endothelial nitric oxide synthase (eNOS) were determined.

resultsPrehypoxic exposure to 10% plasma obtained 6 h after helium inhalation decreased hypoxia-induced cell damage in HUVEC. Cav-1 knockdown in HUVEC abolished this effect.

conclusionsPlasma of healthy volunteers breathing helium protects HUVEC against hypoxic cell damage, possibly involving circulating Cav-1.

Indexed as

Administration, InhalationAdultCaveolin 1Cell HypoxiaCells, CulturedHealthy VolunteersHeliumHumansHuman Umbilical Vein Endothelial CellsMaleMiddle AgedOxygenPlasmaSignal TransductionYoung AdultCAV1 protein, humanCaveolin 1helioxHeliumOxygenCaveolin-1Endothelial conditioningHeliumIschemic preconditioningRemote

Identifiers

PMID31025141
PMCPMC6538579
OpenAlexW2940583803

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.