ArticleJournal of industrial microbiology & biotechnology2019
Efficient production of xylitol by the integration of multiple copies of xylose reductase gene and the deletion of Embden-Meyerhof-Parnas pathway-associated genes to enhance NADPH regeneration in Escherichia coli.
Article in Journal of industrial microbiology & biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 18 citations in OpenAlex.
- Metabolic engineering of Escherichia coli for resveratrol production using food-grade D-xylose as a carbon source.Journal of industrial microbiology & biotechnology · 2026Article
- Current trends in the production of xylitol and paving the way for metabolic engineering in microbes.Biotechnology for biofuels and bioproducts · 2025Review
- Construction of antibiotic-free riboflavin producer inSynthetic and systems biotechnology · 2025Article
- Lignocellulosic xylitol production from corncob using engineeredFrontiers in bioengineering and biotechnology · 2022Article
- Valorisation of xylose to renewable fuels and chemicals, an essential step in augmenting the commercial viability of lignocellulosic biorefineries.Sustainable energy & fuels · 2021Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Cofactor supply is a rate-limiting step in the bioconversion of xylose to xylitol. Strain WZ04 was first constructed by a novel simultaneous deletion-insertion strategy, replacing ptsG, xylAB and ptsF in wild-type Escherichia coli W3110 with three mutated xylose reductase genes (xr) from Neurospora crassa. Then, the pfkA, pfkB, pgi and/or sthA genes were deleted and replaced by xr to investigate the influence of carbon flux toward the pentose phosphate pathway and/or transhydrogenase activity on NADPH generation. The deletion of pfkA/pfkB significantly improved NADPH supply, but minimally influenced cell growth. The effects of insertion position and copy number of xr were examined by a quantitative real-time PCR and a shake-flask fermentation experiment. In a fed-batch fermentation experiment with a 15-L bioreactor, strain WZ51 produced 131.6 g L
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Registered trials
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