ArticleApplied microbiology and biotechnology2019
A TaqMan-probe-based multiplex real-time RT-qPCR for simultaneous detection of porcine enteric coronaviruses.
Article in Applied microbiology and biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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Who cites it
63 citing papers in PubMed, 94 citations in OpenAlex.
- Norovirus inter-species transmission in an ex vivo model is dependent on virus internalization capacity.Emerging microbes & infections · 2026Article
- Epidemiological characteristics of co-infection between porcine epidemic diarrhea virus (PEDV) and other pathogens: a meta-analysis and systematic review.Porcine health management · 2026Article
- A monoclonal antibody-based electrochemical immunosensor for porcine epidemic diarrhea virus.Applied microbiology and biotechnology · 2026Article
- N6-methyladenosine within transmissible gastroenteritis virus genomic RNA inhibits its replication via efficient recognition by RNA sensor RIG-I.Journal of virology · 2026Article
- Isolation, genomic characterization, and pathogenicity of an emerging PEDV variant in Korea related to virulent Chinese strains.Frontiers in veterinary science · 2026Article
- Establishment and application of a droplet digital PCR assay for sensitive detection of porcine deltacoronavirus.BMC veterinary research · 2025Article
- Advancements in Modern Nucleic Acid-Based Multiplex Testing Methodologies for the Diagnosis of Swine Infectious Diseases.Veterinary sciences · 2025Review
- CRISPR/Cas12a-Based One-Tube RT-RAA Assay for PoRV Genotyping.International journal of molecular sciences · 2025Article
- Insights into cross-species infection: Porcine epidemic diarrhea virus infections in the rodent.Virologica Sinica · 2025Article
- A TaqMan probe-based multiplex real-time quantitative pcr for simultaneous detection of kobuvirus, parechovirus B, rosavirus B, and hunnivirus carried by murine rodents and shrews.Virology journal · 2025Article
- Advances in porcine epidemic diarrhea virus research: genome, epidemiology, vaccines, and detection methods.Discover nano · 2025Review
- Development and Immunogenicity Study of Subunit Vaccines Based on Spike Proteins of Porcine Epidemic Diarrhea Virus and Porcine Transmissible Gastroenteritis Virus.Veterinary sciences · 2025Article
- Potassium molybdate blocks APN-dependent coronavirus entry by degrading receptor via PIK3C3-mediated autophagy.Journal of virology · 2025Article
- Molecular characteristics and potential antigenic epitope analysis of porcine epidemic diarrhea virus in China from 2022 to 2025.Frontiers in veterinary science · 2025Article
- Establishment and application of a quadruple RT-qPCR method for simultaneous detection of porcine enteric coronaviruses.Frontiers in veterinary science · 2025Article
- Development and implementation of a TaqMan triplex real-time PCR assay for concurrent detection of pseudorabies virus, porcine teschovirus 1, andFrontiers in veterinary science · 2025Article
- Article
- Development and Validation of RAA-CRISPR/Cas12a-Based Assay for Detecting Porcine Rotavirus.Animals : an open access journal from MDPI · 2024Article
- Isolation and evaluation of the pathogenicity of a hybrid shiga toxin-producing and Enterotoxigenic Escherichia coli in pigs.BMC veterinary research · 2024Article
- Detection of Porcine Circovirus (PCV) Using CRISPR-Cas12a/13a Coupled with Isothermal Amplification.Viruses · 2024Review
3 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Swine enteric coronaviruses are a group of most significant pathogens causing diarrhea in piglets with similar clinical symptoms and pathological changes. To develop a simple, rapid, accurate, and high-throughput detection method for diagnosis and differential diagnosis on swine enteric coronaviruses, specific primers and probes were designed based on the highly conserved regions of transmissible gastroenteritis virus (TGEV) N, porcine epidemic diarrhea virus (PEDV) M, porcine deltacoronavirus (PDCoV) M, and porcine enteric alphacoronavirus (PEAV) N genes respectively. A TaqMan-probe-based multiplex real-time RT-qPCR assay was developed and optimized to simultaneously detect these swine enteric coronaviruses. The results showed that the limit of detection can reach as low as 10 copies in singular real-time RT-qPCR assays and 100 copies in multiplex real-time RT-qPCR assay, with all correlation coefficients (R
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.