ArticleApplications in plant sciences2019
Utilizing herbarium specimens to quantify historical mycorrhizal communities.
Article in Applications in plant sciences, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 28 citations in OpenAlex.
- Herbaria unlock tripartite pollination responses to anthropogenic change.Trends in ecology & evolution · 2026Review
- Temporal and spatial shifts in the foliar fungal endophyte community of Norway spruce (Picea abies) over 150 years in Finland.Environmental microbiome · 2026Article
- Metagenomics Approach for Identification of Arbuscular Mycorrhizal Fungal Sequences in Historical Herbarium Specimens.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Increasing Prevalence of Plant-Fungal Symbiosis Across Two Centuries of Environmental Change.Global change biology · 2025Article
- Ancient Microbiomes as Mirrored by DNA Extracted From Century-Old Herbarium Plants and Associated Soil.Molecular ecology resources · 2025Article
- Back to the future: Using herbarium specimens to isolate nodule-associated bacteria.Ecology and evolution · 2024Article
- Paleomicrobiology: Tracking the past microbial life from single species to entire microbial communities.Microbial biotechnology · 2024Review
- On the potential of Angiosperms353 for population genomic studies.Applications in plant sciences · 2021Article
- The Herbarium 2021 Half-Earth Challenge Dataset and Machine Learning Competition.Frontiers in plant science · 2021Article
- Detection and annotation of plant organs from digitised herbarium scans using deep learning.Biodiversity data journal · 2020Article
- Mining new sources of natural history observations for disease interactions.American journal of botany · 2020Article
- Responses of Rhizosphere Fungal Communities to the Sewage Sludge Application into the Soil.Microorganisms · 2019Article
- Utilizing herbarium specimens to quantify historical mycorrhizal communities.Applications in plant sciences · 2019Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
premise of the studyMycorrhiza are critical to ecosystem functioning, but a lack of historical baseline data limits our understanding of the long-term belowground effects of global change. Herbarium specimens may provide this needed insight. However, it is unknown whether DNA of arbuscular mycorrhizal fungi (AMF) can be reliably extracted from vascular plant specimen roots.
methodsWe sampled roots from herbarium specimens of four herbaceous forest species collected in western Pennsylvania between 1881-2008. Using molecular methods (terminal restriction fragment length polymorphism and sequence analysis), we quantified AMF communities from specimen roots and tested for contamination.
resultsWe successfully amplified AMF DNA from 44% (21/48) of the root but not leaf samples, indicating specimen contamination was negligible. As expected, there were significant differences in AMF composition between plant species ( DISCUSSION: Herbaria have potential as sources of valuable belowground microbial data to answer questions across geographic, temporal, and taxonomic scales otherwise not feasible. Ongoing methodological developments will only magnify this potential. Further tests are needed to determine curatorial practices that maximize this innovative use of herbarium specimens.
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