ArticleFrontiers in immunology2019
Fix Your Membrane Receptor Imaging: Actin Cytoskeleton and CD4 Membrane Organization Disruption by Chemical Fixation.
Article in Frontiers in immunology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
- Quantitative Characterization of Microtubule Ultrastructure Based on Single-Molecule Localization Microscopy.Cytoskeleton (Hoboken, N.J.) · 2026Article
- HIV-1 envelope glycoprotein modulates CXCR4 clustering and dynamics on the T cell membrane.eLife · 2026Article
- Novel fixative solutions with superior preservation of structure and antigenicity are suitable for optical clearing, tissue staining, and flow cytometry.Nagoya journal of medical science · 2026Article
- MERTK coordinates efferocytosis by regulating integrin localization and activation.Journal of cell science · 2026Article
- Cryogenic light microscopy of vitrified samples with angstrom precision.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Histology of the fascial planes: a systematic review of the microstructural foundations of regional anesthesia.Journal of anesthesia, analgesia and critical care · 2025Review
- 3D Microstructural Characterization of Human Deep Fascia Using Optical Projection Tomography, Digital Light Sheet Microscopy, and Magnetic Resonance Microscopy.Microscopy research and technique · 2025Article
- Nonmuscle myosin II regulates presynaptic actin and neuronal mechanobiology in Drosophila.The Journal of cell biology · 2025Article
- Cryo-light microscopy with angstrom precision deciphers structural conformations of PIEZO1 in its native state.Science advances · 2025Article
- Unveiling the native architecture of adult cardiac tissue using the 3D-NaissI method.Cellular and molecular life sciences : CMLS · 2025Article
- Microfluidics-based automatic immunofluorescence staining for single-molecule localization microscopy.Biomedical optics express · 2024Article
- Chemokine receptor distribution on the surface of repolarizing T cells.Biophysical journal · 2024Article
- Direct observation of the effects of chemical fixation in MNT-1 cells: A SE-ADM and Raman study.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- The Impact of Chemical Fixation on the Microanatomy of Mouse Organotypic Hippocampal Slices.eNeuro · 2023Article
- Three-dimensional fine structures in deep fascia revealed by combined use of cryo-fixed histochemistry and low-vacuum scanning microscopy.Scientific reports · 2023Article
- Chemical fixation creates nanoscale clusters on the cell surface by aggregating membrane proteins.Communications biology · 2022Article
- Investigation of Red Blood Cells by Atomic Force Microscopy.Sensors (Basel, Switzerland) · 2022Article
- Ten Approaches That Improve Immunostaining: A Review of the Latest Advances for the Optimization of Immunofluorescence.International journal of molecular sciences · 2022Review
- Structural Configuration of Blood Cell Membranes Determines Their Nonlinear Deformation Properties.BioMed research international · 2022Article
- Diurnal Differences in Intracellular Replication Within Splenic Macrophages Correlates With the Outcome of Pneumococcal Infection.Frontiers in immunology · 2022Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Single-molecule localization microscopy (SMLM) techniques allow near molecular scale resolution (~ 20 nm) as well as precise and robust analysis of protein organization at different scales. SMLM hardware, analytics and probes have been the focus of a variety of studies and are now commonly used in laboratories across the world. Protocol reliability and artifact identification are increasingly seen as important aspects of super-resolution microscopy. The reliability of these approaches thus requires in-depth evaluation so that biological findings are based on solid foundations. Here we explore how different fixation approaches that disrupt or preserve the actin cytoskeleton affect membrane protein organization. Using CD4 as a model, we show that fixation-mediated disruption of the actin cytoskeleton correlates with changes in CD4 membrane organization. We highlight how these artifacts are easy to overlook and how careful sample preparation is essential for extracting meaningful results from super-resolution microscopy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.