ArticleScience (New York, N.Y.)2019
Long-term functional maintenance of primary human hepatocytes in vitro.
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Article in Science (New York, N.Y.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 122 papers, 2 of them syntheses that pooled it.
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122citing papers in PubMed, 2 pooled it
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Who cites it
122 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Rodent model of metabolic dysfunction-associated fatty liver disease: a systematic review.Journal of gastroenterology and hepatology · 2025Pooled it
- A comprehensive transcriptomic comparison of hepatocyte model systems improves selection of models for experimental use.Communications biology · 2022Pooled it
- Dual HBV cccDNA-linked HiBiT reporter hepatocyte models for screening of candidate cccDNA modulators.Virulence · 2026Article
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- Six-compound cocktail for maturation of human pluripotent stem cell-derived liver spheroids for toxicity prediction.Nature communications · 2026Article
- Identification of Cyclin L1 as a Host Factor Regulating Hepatitis B Virus Replication.Viruses · 2026Article
- A Dynamic 3D Human Liver Sinusoid Model for Mechanistic Interrogation of Fontan-Associated Liver Disease.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Generation of proliferative hESC-derived grape-clustered hepatocyte organoids with multipolar architecture as regenerative counterpart via synergy of YAP and IGF2 pathways.Cell death & disease · 2026Article
- Preclinical efficacy and safety evaluation of human embryonic stem cell-derived functional hepatocytes for a clinical trial in acute liver failure.Stem cell research & therapy · 2026Article
- Rapid expansion of primary human vocal fold epithelial cells via targeted pathway inhibition and anchorage-independent sphere culture.Cell reports methods · 2026Article
- Three-Dimensional Culture of Primary Hepatocytes in a Single-Cell Layer on Poly(vinyl alcohol) Nanofibrous Membrane.International journal of molecular sciences · 2026Article
- Applications and limitations of pluripotent stem cell-derived liver organoids.Chinese medical journal · 2026Review
- A small molecule strategy with forskolin and p38 inhibitor for serum-free muscle stem cell expansion.NPJ science of food · 2026Article
- Expression and function of CYP enzymes and hepatobiliary transporters in an improved long-term sandwich culture hepatocyte model.Scientific reports · 2026Article
- Inhibition of Wnt signaling in primary human hepatocytes promotes Plasmodium falciparum liver stage development.PLoS pathogens · 2025Article
- Generation and applications of an expandable and mature hiPSC-derived liver organoid.Acta pharmaceutica Sinica. B · 2025Article
- Expansion of human hepatocytes and their application in three-dimensional culture and genetic manipulation.Nature protocols · 2025Article
- Centimeter-Scale Self-Assembling Tendon Organoids Drive Tissue Regeneration.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- The validation of quality attributes in Primary Human Hepatocytes Standard.Cell regeneration (London, England) · 2025Article
- A patient-derived organoid model captures fetal-like plasticity in colorectal cancer.Cell research · 2025Article
62 more citing papers are in PubMed but not listed here.
Corrections and comments
- Commented on byNext generation2020
Authors and funding
27 authors.
Chengang XiangSchool of Basic Medical Sciences, State Key Laboratory of Natural and Biomimetic Drugs, Peking University Health Science Center and the MOE Key Laboratory of Cell Proliferation and Differentiation, College of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100191, China.ORCID 0000-0001-8630-6446
Yuanyuan DuSchool of Basic Medical Sciences, State Key Laboratory of Natural and Biomimetic Drugs, Peking University Health Science Center and the MOE Key Laboratory of Cell Proliferation and Differentiation, College of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100191, China.ORCID 0000-0002-8293-328X
Gaofan MengSchool of Basic Medical Sciences, State Key Laboratory of Natural and Biomimetic Drugs, Peking University Health Science Center and the MOE Key Laboratory of Cell Proliferation and Differentiation, College of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100191, China.ORCID 0000-0001-5115-2176
Liew Soon YiSchool of Basic Medical Sciences, State Key Laboratory of Natural and Biomimetic Drugs, Peking University Health Science Center and the MOE Key Laboratory of Cell Proliferation and Differentiation, College of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100191, China.
Shicheng SunSchool of Basic Medical Sciences, State Key Laboratory of Natural and Biomimetic Drugs, Peking University Health Science Center and the MOE Key Laboratory of Cell Proliferation and Differentiation, College of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100191, China.
Nan SongSchool of Basic Medical Sciences, State Key Laboratory of Natural and Biomimetic Drugs, Peking University Health Science Center and the MOE Key Laboratory of Cell Proliferation and Differentiation, College of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100191, China.
Xiaonan ZhangShanghai Public Health Clinical Center, Shanghai 201508, China.ORCID 0000-0002-1286-7280
Yiwei XiaoSchool of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China.ORCID 0000-0003-0677-0746
Jie WangSchool of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China.
Zhigang YiKey Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai 200032, China.
Yifang LiuCenter for Bioinformatics, Peking University, Beijing 100871, China.
Bingqing XieState Key Laboratory of Chemical Oncogenomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, Shenzhen 518055, Guangdong, China.ORCID 0000-0001-9433-1641
Min WuShanghai Public Health Clinical Center, Shanghai 201508, China.
Jun ShuKey Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai 200032, China.
Da SunState Key Laboratory of Chemical Oncogenomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, Shenzhen 518055, Guangdong, China.
Jun JiaSchool of Basic Medical Sciences, State Key Laboratory of Natural and Biomimetic Drugs, Peking University Health Science Center and the MOE Key Laboratory of Cell Proliferation and Differentiation, College of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100191, China.
Zhen LiangState Key Laboratory of Chemical Oncogenomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, Shenzhen 518055, Guangdong, China.
Dong SunSchool of Basic Medical Sciences, State Key Laboratory of Natural and Biomimetic Drugs, Peking University Health Science Center and the MOE Key Laboratory of Cell Proliferation and Differentiation, College of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100191, China.
Yanxiang HuangYouan Hospital, Beijing 100069, China.
Yan ShiSchool of Basic Medical Sciences, State Key Laboratory of Natural and Biomimetic Drugs, Peking University Health Science Center and the MOE Key Laboratory of Cell Proliferation and Differentiation, College of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100191, China.ORCID 0000-0003-3650-1053
Jun XuSchool of Basic Medical Sciences, State Key Laboratory of Natural and Biomimetic Drugs, Peking University Health Science Center and the MOE Key Laboratory of Cell Proliferation and Differentiation, College of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100191, China.
Fengmin LuSchool of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China.
Cheng LiCenter for Bioinformatics, Peking University, Beijing 100871, China.
Kuanhui XiangSchool of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China.
Zhenghong YuanKey Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai 200032, China. hongkui_deng@pku.edu.cn lsc620213@aliyun.com zhyuan@shmu.edu.cn.
Shichun LuDepartment of Hepatobiliary Surgery, Chinese PLA General Hospital, Beijing 100853, China. hongkui_deng@pku.edu.cn lsc620213@aliyun.com zhyuan@shmu.edu.cn.ORCID 0000-0003-3278-0534
Hongkui DengSchool of Basic Medical Sciences, State Key Laboratory of Natural and Biomimetic Drugs, Peking University Health Science Center and the MOE Key Laboratory of Cell Proliferation and Differentiation, College of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100191, China. hongkui_deng@pku.edu.cn lsc620213@aliyun.com zhyuan@shmu.edu.cn.ORCID 0000-0003-3045-4428
Funding
No grant is acknowledged in the PubMed record.
Abstract
The maintenance of terminally differentiated cells, especially hepatocytes, in vitro has proven challenging. Here we demonstrated the long-term in vitro maintenance of primary human hepatocytes (PHHs) by modulating cell signaling pathways with a combination of five chemicals (5C). 5C-cultured PHHs showed global gene expression profiles and hepatocyte-specific functions resembling those of freshly isolated counterparts. Furthermore, these cells efficiently recapitulated the entire course of hepatitis B virus (HBV) infection over 4 weeks with the production of infectious viral particles and formation of HBV covalently closed circular DNA. Our study demonstrates that, with a chemical approach, functional maintenance of PHHs supports long-term HBV infection in vitro, providing an efficient platform for investigating HBV cell biology and antiviral drug screening.
Indexed as
Antiviral AgentsDNA, CircularDNA, ViralDrug Evaluation, PreclinicalHepatitis B virusHepatocytesHumansPrimary Cell CultureTranscriptomeVirionVirus CultivationAntiviral AgentsDNA, CircularDNA, Viral
Identifiers
PMID
31023926What OpenQuestion holds
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.