Evidence map›Paper›PMID 31023535›Full record

ArticleBiophysical journal2019

The Fluidity of Phosphocholine and Maltoside Micelles and the Effect of CHAPS.

Marissa Kieber, Tomihiro Ono, Ryan C Oliver, Sarah B Nyenhuis, D Peter Tieleman, Linda Columbus

Erratum issuedOpen access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biophysical journal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Marissa KieberDepartment of Chemistry, University of Virginia, Charlottesville, Virginia.
Tomihiro OnoDepartment of Chemistry, University of Virginia, Charlottesville, Virginia.
Ryan C OliverDepartment of Chemistry, University of Virginia, Charlottesville, Virginia.
Sarah B NyenhuisDepartment of Chemistry, University of Virginia, Charlottesville, Virginia.
D Peter TielemanDepartment of Biological Sciences and Centre for Molecular Simulation, University of Calgary, Calgary, Alberta, Canada.
Linda ColumbusDepartment of Chemistry, University of Virginia, Charlottesville, Virginia. Electronic address: columbus@virginia.edu.
University of Virginia · USUniversity of Calgary · CA

Funding

Structure and dynamics of bacterial membrane protein - receptor interactionsR01GM087828 · NIGMS · UNIVERSITY OF VIRGINIA · PI COLUMBUS, LINDA M · 2009 to 2018
$2.7M
NIGMS NIH HHS R01 GM087828
6 · The paper itself

Abstract

The dynamics of phosphocholine and maltoside micelles, detergents frequently used for membrane protein structure determination, were investigated using electron paramagnetic resonance of spin probes doped into the micelles. Specifically, phosphocholines are frequently used detergents in NMR studies, and maltosides are frequently used in x-ray crystallography structure determination. Beyond the structural and electrostatic differences, this study aimed to determine whether there are differences in the local chain dynamics (i.e., fluidity). The nitroxide probe rotational dynamics in longer chain detergents is more restricted than in shorter chain detergents, and maltoside micelles are more restricted than phosphocholine micelles. Furthermore, the micelle microviscosity can be modulated with mixtures, as demonstrated with mixtures of 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate with n-dodecylphosphocholine, n-tetradecylphosphocholine, n-decyl-β-D-maltoside, or n-dodecyl-β-D-maltoside. These results indicate that observed differences in membrane protein stability in these detergents could be due to fluidity in addition to the already determined structural differences.

Indexed as

Membrane FluidityMicellesCholic AcidsMaltoseOxygenPhosphorylcholine3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonateCholic AcidsMaltoseMicellesOxygenPhosphorylcholine

Identifiers

PMID31023535
PMCPMC6506624
OpenAlexW2926149703

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.