ArticleMalaria journal2019
Efficient synchronization of Plasmodium knowlesi in vitro cultures using guanidine hydrochloride.
Article in Malaria journal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Beyond the mouse: organoids, spheroids, and organs-on-chips as the (inevitable) future of malaria research?Malaria journal · 2026Review
- Comparative evaluation of synchronization methods for Plasmodium falciparum: Efficiency, practicality and experimental applicability.Parasites, hosts and diseases · 2026Article
- Red blood cell signaling is functionally conserved iniScience · 2024Article
- Melatonin as a Circadian Marker forInternational journal of molecular sciences · 2024Review
- Guanidinium Chloride-Induced Haemolysis Assay to Measure New Permeation Pathway Functionality in Rodent MalariaBiomolecules · 2024Article
- Leishmanicidal Activity of Guanidine Derivatives againstTropical medicine and infectious disease · 2023Article
- Fluorescent Nitrogen-Doped Carbon Dots for Label Live Elder Blood-StageMolecules (Basel, Switzerland) · 2022Article
- In-depth biological analysis of alteration in Plasmodium knowlesi-infected red blood cells using a noninvasive optical imaging technique.Parasites & vectors · 2022Article
- Article
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Authors and funding
6 authors.
Funding
Abstract
backgroundLong-term in vitro culture of blood stage Plasmodium parasites invariably leads to asynchronous parasite development. The most often used technique to synchronize Plasmodium falciparum culture is sorbitol treatment, which differentially induces osmotic lysis of trophozoite- and schizont-infected red blood cells due to presence of the new permeation pathways in the membranes of these cells. However, sorbitol treatment does not work well when used to synchronize the culture-adapted Plasmodium knowlesi A1-H.1 line.
methodsA number of common solutes were tested in lieu of sorbitol for synchronization of P. knowlesi A1-H.1 ring stage.
resultsGuanidine hydrochloride was found to selectively lyse trophozoite- and schizont-infected red blood cells, yielding highly synchronous and viable rings.
conclusionsA method for synchronization of P. knowlesi in human red blood cells was developed. Requiring only common laboratory reagents, this method is simple and should be applicable to most laboratory settings.
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