ArticlePlant biotechnology journal2019
Characterizing standard genetic parts and establishing common principles for engineering legume and cereal roots.
Article in Plant biotechnology journal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 38 citations in OpenAlex.
- The saltmarsh cordgrass Spartina anglica has novel genomic features that contribute to abiotic stress tolerance.Journal of experimental botany · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- From Structure to Function of Promoters and 5'UTRs in Maize.International journal of molecular sciences · 2026Review
- Synthetic promoter design in plants: integration of computational and experimental approaches.Frontiers in plant science · 2026Review
- Toward a monocot SynBio toolkit: assessing regulatory element performance and eudicot compatibility.Plant cell reports · 2025Article
- A simple and efficient TALEN system for genome editing in plants.Plant molecular biology · 2025Article
- Ectopic expression of the GRAS-type transcriptional regulatorFrontiers in plant science · 2024Article
- Impact of Intron and Retransformation on Transgene Expression in Leaf and Fruit Tissues of Field-Grown Pear Trees.International journal of molecular sciences · 2023Article
- Sourcing Phage-Encoded Terminators Using ONT-cappable-seq for SynBio Applications inACS synthetic biology · 2023Article
- CIPK-B is essential for salt stress signalling in Marchantia polymorpha.The New phytologist · 2023Article
- Plant Promoters and Terminators for High-Precision Bioengineering.Biodesign research · 2023Review
- Nutrient regulation of lipochitooligosaccharide recognition in plants via NSP1 and NSP2.Nature communications · 2022Article
- Design and Analysis of Native Photorespiration Gene Motifs of Promoter Untranslated Region Combinations Under Short Term Abiotic Stress Conditions.Frontiers in plant science · 2022Article
- A Ratiometric Dual Color Luciferase Reporter for Fast Characterization of Transcriptional Regulatory Elements in Plants.ACS synthetic biology · 2021Article
- Article
- A bipartite transcription factor module controlling expression in the bundle sheath of Arabidopsis thaliana.Nature plants · 2020Article
- Turning Up the Temperature on CRISPR: Increased Temperature Can Improve the Editing Efficiency of Wheat Using CRISPR/Cas9.Frontiers in plant science · 2020Article
- Reconfiguring Plant Metabolism for Biodegradable Plastic Production.Biodesign research · 2020Review
- A modular steroid-inducible gene expression system for use in rice.BMC plant biology · 2019Article
- The Rooting of Stem Cuttings and the Stability ofPlants (Basel, Switzerland) · 2019Article
Corrections and comments
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Authors and funding
12 authors at 2 institutions in 2 countries.
Funding
Abstract
Plant synthetic biology and cereal engineering depend on the controlled expression of transgenes of interest. Most engineering in plant species to date has relied heavily on the use of a few, well-established constitutive promoters to achieve high levels of expression; however, the levels of transgene expression can also be influenced by the use of codon optimization, intron-mediated enhancement and varying terminator sequences. Most of these alternative approaches for regulating transgene expression have only been tested in small-scale experiments, typically testing a single gene of interest. It is therefore difficult to interpret the relative importance of these approaches and to design engineering strategies that are likely to succeed in different plant species, particularly if engineering multigenic traits where the expression of each transgene needs to be precisely regulated. Here, we present data on the characterization of 46 promoters and 10 terminators in Medicago truncatula, Lotus japonicus, Nicotiana benthamiana and Hordeum vulgare, as well as the effects of codon optimization and intron-mediated enhancement on the expression of two transgenes in H. vulgare. We have identified a core set of promoters and terminators of relevance to researchers engineering novel traits in plant roots. In addition, we have shown that combining codon optimization and intron-mediated enhancement increases transgene expression and protein levels in barley. Based on our study, we recommend a core set of promoters and terminators for broad use and also propose a general set of principles and guidelines for those engineering cereal species.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.