Evidence map›Paper›PMID 31021258›Full record

ArticleThe CRISPR journal2018

TIE: A Method to Electroporate Long DNA Templates into Preimplantation Embryos for CRISPR-Cas9 Gene Editing.

Hooman Bagheri, Hana Friedman, Harry Shao, Yumaine Chong, Chiu-An Lo, Farida Emran, Ibrahim Kays, Xiang-Jiao Yang, Ellis Cooper, Brian E Chen and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The CRISPR journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Recent Advances in the Production of Genome-Edited Rats.International journal of molecular sciences · 2022
    Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Hooman Bagheri1 Laboratory of Developmental Biology, McGill University , Montreal, Quebec, Canada.
Hana Friedman1 Laboratory of Developmental Biology, McGill University , Montreal, Quebec, Canada.
Harry Shao1 Laboratory of Developmental Biology, McGill University , Montreal, Quebec, Canada.
Yumaine Chong5 Department of Physiology, McGill University , Montreal, Quebec, Canada.
Chiu-An Lo6 Centre for Research in Neuroscience, Research Institute of the McGill University Health Centre , Montreal, Quebec, Canada.
Farida Emran6 Centre for Research in Neuroscience, Research Institute of the McGill University Health Centre , Montreal, Quebec, Canada.
Ibrahim Kays6 Centre for Research in Neuroscience, Research Institute of the McGill University Health Centre , Montreal, Quebec, Canada.
Xiang-Jiao Yang7 Department of Biochemistry, McGill University , Montreal, Quebec, Canada.
Ellis Cooper5 Department of Physiology, McGill University , Montreal, Quebec, Canada.
Brian E Chen6 Centre for Research in Neuroscience, Research Institute of the McGill University Health Centre , Montreal, Quebec, Canada.
Katherine Siminovitch8 Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital , Toronto, Ontario, Canada .
Alan Peterson1 Laboratory of Developmental Biology, McGill University , Montreal, Quebec, Canada.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Precise genome editing using CRISPR typically requires delivery of guide RNAs, Cas9 endonuclease, and DNA repair templates. Both microinjection and electroporation effectively deliver these components into mouse zygotes provided the DNA template is an oligonucleotide of only a few hundred base pairs. However, electroporation completely fails with longer double-stranded DNAs leaving microinjection as the only delivery option. Here, we overcome this limitation by first injecting all CRISPR components, including long plasmid-sized DNA templates, into the sub-zona pellucida space. There they are retained, supporting subsequent electroporation. We show that this simple and well-tolerated method achieves intracellular reagent concentrations sufficient to effect precise gene edits.

Identifiers

PMID31021258
PMCPMC6636866

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.