Evidence map›Paper›PMID 31021092›Full record

ArticleThe journal of physical chemistry. B2019

Polarized Two-Photon Absorption and Heterogeneous Fluorescence Dynamics in NAD(P)H.

Thomas S Blacker, Nick Nicolaou, Michael R Duchen, Angus J Bain

Abstract read
In one paragraph

Article in The journal of physical chemistry. B, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Thomas S BlackerDepartment of Physics & Astronomy , University College London , Gower Street , London WC1E 6BT , United Kingdom.
Nick NicolaouDepartment of Physics & Astronomy , University College London , Gower Street , London WC1E 6BT , United Kingdom.
Michael R DuchenResearch Department of Cell & Developmental Biology , University College London , Gower Street , London WC1E 6BT , United Kingdom.
Angus J BainDepartment of Physics & Astronomy , University College London , Gower Street , London WC1E 6BT , United Kingdom.ORCID 0000-0001-8705-8536

Funding

Biotechnology and Biological Sciences Research Council BB/L020874/1
6 · The paper itself

Abstract

Two-photon absorption (2PA) finds widespread application in biological systems, which frequently exhibit heterogeneous fluorescence decay dynamics corresponding to multiple species or environments. By combining polarized 2PA with time-resolved fluorescence intensity and anisotropy decay measurements, we show how the two-photon transition tensors for the components of a heterogeneous population can be separately determined, allowing structural differences between the two fluorescent states of the redox cofactor NAD(P)H to be identified. The results support the view that the two states correspond to alternate configurations of the nicotinamide ring, rather than folded and extended conformations of the entire molecule.

Identifiers

PMID31021092
PMCPMC6558641

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.