ArticleMolecular biotechnology2019
A Novel Approach to Deliver a Mycobacterium avium subsp. paratuberculosis Antigen in Eukaryotic Cells.
Article in Molecular biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed, 6 citations in OpenAlex.
- Modeling Paratuberculosis in Laboratory Animals, Cells, or Tissues: A Focus on Their Applications for Pathogenesis, Diagnosis, Vaccines, and Therapy Studies.Animals : an open access journal from MDPI · 2023Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
This study was aimed to express and deliver a Mycobacterium avium subsp. paratuberculosis antigen to macrophages using salmonella as carrier. The coding sequence of a fibronectin attachment protein which is expressed by Mycobacterium avium subsp. paratuberculosis was cloned into pcDNA3.1 (+) plasmid. The construct was introduced into the attenuated Salmonella typhimurium strain SL7207 (ΔhisG, ΔaroA) as carrier. In order to evaluate the delivery capacity of Salmonella and gene expression by antigen-presenting cells, the THP-1 derived macrophages were infected with the salmonella carrier. SDS-PAGE and western blot analysis showed the successful delivery and expression of targeted gene in THP-1 cell line. Although, in vitro stimulation of peripheral blood mononuclear cells with Salmonella containing plasmid did not trigger IFNγ production significantly. But it seems that this carrier can increase plasmid uptake and antigen expression by host intestinal antigen-presenting cells after mucosal administration. So, the construct can be used for further in vivo studies on the Salmonella carrier's efficiency in mycobacterial DNA vaccines.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.