Evidence map›Paper›PMID 31019379›Full record

ArticleBiological procedures online2019

A Cautionary Tale: Endogenous Biotinylated Proteins and Exogenously-Introduced Protein A Cause Antibody-Independent Artefacts in Western Blot Studies of Brain-Derived Proteins.

Marianne K O Grant, Samantha L Shapiro, Karen H Ashe, Peng Liu, Kathleen R Zahs

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biological procedures online, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
0.7field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 13 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Marianne K O Grant1Departments of Neurology, University of Minnesota, Minneapolis, MN 55455 USA.
Samantha L Shapiro1Departments of Neurology, University of Minnesota, Minneapolis, MN 55455 USA.
Karen H Ashe1Departments of Neurology, University of Minnesota, Minneapolis, MN 55455 USA.
Peng Liu1Departments of Neurology, University of Minnesota, Minneapolis, MN 55455 USA.
Kathleen R Zahs1Departments of Neurology, University of Minnesota, Minneapolis, MN 55455 USA.ORCID 0000-0002-0617-3370
University of Minnesota · US

Funding

Women's CancerP30CA077598 · NCI · UNIVERSITY OF MINNESOTA TWIN CITIES · PI Douglas Yee · 1998 to 2026
$100.4M
TOWARD A MODEL OF ALZHEIMERS DISEASER01NS033249 · NINDS · UNIVERSITY OF MINNESOTA TWIN CITIES · PI ASHE, KAREN H · 1994 to 2013
$6.5M
Specific amyloid-beta oligomer aptamersRC1AG035870 · NIA · UNIVERSITY OF MINNESOTA · PI ASHE, KAREN H · 2009 to 2010
$739k
NCI NIH HHS P30 CA077598NIA NIH HHS RC1 AG035870NINDS NIH HHS R01 NS033249
6 · The paper itself

Abstract

Antibodies are commonly used to detect or isolate proteins from biological samples. Much attention has been paid to the potential for poorly-characterized antibodies to lead to misleading results, but

Indexed as

ArtefactBiotinProtein AWestern blotting

Identifiers

PMID31019379
PMCPMC6474067
OpenAlexW2943778498

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.