ArticleScientific reports2019
A fast, reliable and sample-sparing method to identify fibre types of single muscle fibres.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Effects of eccentric, concentric and eccentric/concentric training on muscle function and mass, functional performance, cardiometabolic health, quality of life and molecular adaptations of skeletal muscle in COPD patients: a multicentre randomised trial.BMC pulmonary medicine · 2022Trial
- Fibre Type-Specific Proteomics Reveals Shared and Distinct Skeletal Muscle Adaptations to Resistance Training and BetaJournal of cachexia, sarcopenia and muscle · 2026Article
- Exercise attenuates stress-related signaling as sensed by higher phosphorylation of small heat shock proteins in skeletal muscle from older individuals.Journal of sport and health science · 2025Article
- Integrated fibre-specific methylome and proteome profiling of human skeletal muscle across males and females with fibre-type deconvolution.Skeletal muscle · 2025Article
- Fiber-specific differences in protein content of pathways related to mTORC1 signaling and oxidative metabolism in individuals with obesity.Scientific reports · 2025Article
- Endurance exercise with reduced muscle glycogen content influences substrate utilization and attenuates acute mTORC1- and autophagic signaling in human type I and type II muscle fibers.Skeletal muscle · 2025Article
- Fibre-specific mitochondrial protein abundance is linked to resting and post-training mitochondrial content in the muscle of men.Nature communications · 2024Article
- Muscle fibre size and myonuclear positioning in trained and aged humans.Experimental physiology · 2024Article
- Predicting myosin heavy chain isoform from postdissection fiber length in human skeletal muscle fibers.American journal of physiology. Cell physiology · 2024Article
- THRIFTY-A High-throughput Single Muscle Fiber Typing Method Based on Immunofluorescence Detection.Bio-protocol · 2023Article
- THRIFTY: a novel high-throughput method for rapid fibre type identification of isolated skeletal muscle fibres.The Journal of physiology · 2022Article
- Skeletal muscle cell-specific differences in type 2 diabetes.Cellular and molecular life sciences : CMLS · 2022Article
- Loss of α-actinin-3 during human evolution provides superior cold resilience and muscle heat generation.American journal of human genetics · 2021Article
- Skeletal Muscle Adaptations and Performance Outcomes Following a Step and Exponential Taper in Strength Athletes.Frontiers in physiology · 2021Article
- Effects of high-intensity intermittent exercise on the contractile properties of human type I and type II skeletal muscle fibers.Journal of applied physiology (Bethesda, Md. : 1985) · 2020Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many skeletal muscle proteins are present in a cell-specific or fibre-type dependent manner. Stimuli such as exercise, aging, and disease have been reported to result in fibre-specific responses in protein abundances. Thus, fibre-type-specific determination of the content of specific proteins provides enhanced mechanistic understanding of muscle physiology and biochemistry compared with typically performed whole-muscle homogenate analyses. This analysis, however, is laborious and typically not performed. We present a novel dot blotting method for easy and rapid determination of skeletal muscle fibre type based on myosin heavy chain (MHC) isoform presence. Requiring only small amounts of starting muscle tissue (i.e., 2-10 mg wet weight), muscle fibre type is determined in one-tenth of a 1-3-mm fibre segment, with the remainder of each segment pooled with fibre segments of the same type (I or II) for subsequent protein quantification by western blotting. This method, which we validated using standard western blotting, is much simpler and cheaper than previous methods and is adaptable for laboratories routinely performing biochemical analyses. Use of dot blotting for fibre typing will facilitate investigations of fibre-specific responses to diverse stimuli, which will advance our understanding of skeletal muscle physiology and biochemistry.
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