ArticleThe Journal of reproduction and development2019
The necessity of ZSCAN4 for preimplantation development and gene expression of bovine embryos.
Article in The Journal of reproduction and development, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 13 citations in OpenAlex.
- Article
- Developmental origins of pregnancy loss in cattle.Animal reproduction · 2026Article
- Knockdown of Y-box-binding protein 2 induces mitochondrial dysfunction to interrupt zygotic genome activation in porcine embryos.Journal of animal science and technology · 2025Article
- Oleanolic acid improves the in vitro developmental competence of early porcine embryos by reducing oxidative stress and ameliorating mitochondrial function.Animal bioscience · 2025Article
- Genes as Genome Stabilizers in Pluripotent Stem Cells.Advances in experimental medicine and biology · 2025Review
- SINE-Associated LncRNA SAWPA Regulates Porcine Zygotic Genome Activation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- The Role of BDNF, YBX1, CENPF, ZSCAN4, TEAD4, GLIS1 and USF1 in the Activation of the Embryonic Genome in Bovine Embryos.International journal of molecular sciences · 2023Review
- Article
- Pluripotent Core in Bovine Embryos: A Review.Animals : an open access journal from MDPI · 2022Review
- Arginine Regulates Zygotic Genome Activation in Porcine Embryos Under Nutrition Restriction.Frontiers in veterinary science · 2022Article
- Chromatin role in early programming of embryos.Animal frontiers : the review magazine of animal agriculture · 2021Article
- Chromatin remodeling in bovine embryos indicates species-specific regulation of genome activation.Nature communications · 2020Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Zinc finger and SCAN domain containing 4 (Zscan4) is a gene that is specifically expressed during zygotic genome activation (ZGA) in mouse preimplantation embryos, and a reduction of Zscan4 transcripts leads to developmental failure. In mouse embryonic stem cells (ESCs), Zscan4 is expressed transiently in as little as 1-5% of the cell population. Zscan4 has also been shown to enhance the efficiency of mouse induced pluripotent stem cells (iPSCs) generation and their quality. Although ZSCAN4 plays important roles in murine embryos and stem cells, its expression and role in bovine embryos is unknown. This study examines ZSCAN4 transcripts in bovine embryos at various developmental stages and attempts to elucidate the functions of ZSCAN4 during bovine preimplantation development. ZSCAN4 transcripts were found to be upregulated at the 8- and 16-cell stages. We next attempted ZSCAN4 downregulation in bovine early embryos by RNA interference and evaluated developmental competency and transcripts levels of genes involved in ZGA and iPSCs generation. Although the bovine embryos injected with ZSCAN4-siRNA could develop to the 8-cell stage, very few were developing beyond the 16-cell stage. PIWIL2 expression was reduced in ZSCAN4 downregulated embryos. It is possible that ZSCAN4 downregulated embryos fail to regulate gene expression during ZGA. Our results indicate that ZSCAN4 is an important factor for the preimplantation development of bovine embryos.
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