ArticleGenome biology2019
Linking CRISPR-Cas9 interference in cassava to the evolution of editing-resistant geminiviruses.
Article in Genome biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 80 papers.
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Who cites it
80 citing papers in PubMed, 186 citations in OpenAlex.
- Ecology and evolutionary dynamics of the A-strain maize streak virus and the decade-long strategies targeting streak-resistant maize.Virus research · 2026Review
- Mutation of AV2 Start Codon Impairs the Infectivity of a Bipartite Begomovirus Under High Temperature.The plant pathology journal · 2026Article
- Evaluation of CRISPR/Cas9 based single and multiplex gRNAs against ChiLCV in tobacco.Journal, genetic engineering & biotechnology · 2026Article
- Advances in CRISPR Plant Applications.International journal of molecular sciences · 2026Review
- Genome editing‑based strategies to combat geminiviruses: CRISPR/Cas9 and emerging high‑fidelity tools.Archives of microbiology · 2026Review
- Geminiviral-CR-gRNA expressed in cowpea efficiently edited MYMV and MYMIV genome to provide resistance against cowpea yellow mosaic disease without hampering plant growth and yield.BMC plant biology · 2026Article
- CRISPR-Cas technologies for precision genome editing in plants: advances, applications, and future perspectives.Frontiers in plant science · 2026Review
- Progress and Impacts of "Omics" Technologies in Understanding the Drought Response in Cassava: Adoption for Food Security in Africa.Plant-environment interactions (Hoboken, N.J.) · 2025Review
- Whitefly transmitted begomovirus infection in plant and host response: a review.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Review
- Potential for Duplexed, In-Tandem gRNA-Mediated Suppression of Two Essential Genes of Tomato Leaf Curl New Delhi Virus in Crop Plants.Pathogens (Basel, Switzerland) · 2025Article
- CRISPR-mediated genome editing of wheat for enhancing disease resistance.Frontiers in genome editing · 2025Review
- Applications and Prospects of CRISPR/Cas9 Technology in the Breeding of Major Tropical Crops.Plants (Basel, Switzerland) · 2024Review
- CRISPR/Cas: An Emerging Toolbox for Engineering Virus Resistance in Plants.Plants (Basel, Switzerland) · 2024Review
- Geminiviruses and Food Security: A Molecular Genetics Perspective for Sustainable Agriculture in Africa.Plants (Basel, Switzerland) · 2024Review
- Cassava Breeding and Cultivation Challenges in Thailand: Past, Present, and Future Perspectives.Plants (Basel, Switzerland) · 2024Review
- Advanced Biotechnological Interventions in Mitigating Drought Stress in Plants.Plants (Basel, Switzerland) · 2024Review
- Review
- Advances and Prospects of Virus-Resistant Breeding in Tomatoes.International journal of molecular sciences · 2023Review
- Article
- Targeted mutagenesis with sequence-specific nucleases for accelerated improvement of polyploid crops: Progress, challenges, and prospects.The plant genome · 2023Review
20 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundGeminiviruses cause damaging diseases in several important crop species. However, limited progress has been made in developing crop varieties resistant to these highly diverse DNA viruses. Recently, the bacterial CRISPR/Cas9 system has been transferred to plants to target and confer immunity to geminiviruses. In this study, we use CRISPR-Cas9 interference in the staple food crop cassava with the aim of engineering resistance to African cassava mosaic virus, a member of a widespread and important family (Geminiviridae) of plant-pathogenic DNA viruses.
resultsOur results show that the CRISPR system fails to confer effective resistance to the virus during glasshouse inoculations. Further, we find that between 33 and 48% of edited virus genomes evolve a conserved single-nucleotide mutation that confers resistance to CRISPR-Cas9 cleavage. We also find that in the model plant Nicotiana benthamiana the replication of the novel, mutant virus is dependent on the presence of the wild-type virus.
conclusionsOur study highlights the risks associated with CRISPR-Cas9 virus immunity in eukaryotes given that the mutagenic nature of the system generates viral escapes in a short time period. Our in-depth analysis of virus populations also represents a template for future studies analyzing virus escape from anti-viral CRISPR transgenics. This is especially important for informing regulation of such actively mutagenic applications of CRISPR-Cas9 technology in agriculture.
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