ArticleMicrobial cell factories2019
High-titer production of 13R-manoyl oxide in metabolically engineered Saccharomyces cerevisiae.
Article in Microbial cell factories, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 27 citations in OpenAlex.
- Engineered microorganisms unlock a new era of diterpenoid biomanufacturing: recent advances and future perspectives.Bioresources and bioprocessing · 2026Review
- Heterologous Production of Forskolin in Tobacco (Nicotiana tabacum) via Glandular Trichome Specific Engineering and Metabolic Flux Redirection.Plant biotechnology journal · 2026Article
- Strangers in a foreign land: 'Yeastizing' plant enzymes.Microbial biotechnology · 2024Review
- Two-Phase Fermentation Systems for Microbial Production of Plant-Derived Terpenes.Molecules (Basel, Switzerland) · 2024Review
- Pseudomonas putida as a platform for medium-chain length α,ω-diol production: Opportunities and challenges.Microbial biotechnology · 2024Article
- Improved production of TaxolMicrobial cell factories · 2023Article
- Systematic Mining and Evaluation of the Sesquiterpene Skeletons as High Energy Aviation Fuel Molecules.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Metabolic Engineering ofFrontiers in bioengineering and biotechnology · 2022Article
- Tailored biosynthesis of gibberellin plant hormones in yeast.Metabolic engineering · 2021Article
- Progress in heterologous biosynthesis of forskolin.Journal of industrial microbiology & biotechnology · 2021Review
- Protein engineering strategies for microbial production of isoprenoids.Metabolic engineering communications · 2020Review
- Harnessing sub-organelle metabolism for biosynthesis of isoprenoids in yeast.Synthetic and systems biotechnology · 2020Review
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
backgroundDiterpenoids are a large class of natural products with complex structures and broad commercial applications as food additives, important medicines, and fragrances. However, their low abundance in plants and high structural complexity limit their applications. Therefore, it is important to create an efficient diterpenoid-producing yeast cell factory of the production of various high-value diterpenoid compounds in a cost-effective manner
resultsIn this study, 13R-manoyl oxide (13R-MO; 2.31 mg/L) was produced by expressing CfTPS2 and CfTPS3 from Coleus forskohlii in Saccharomyces cerevisiae. The 13R-MO titer was increased by 142-fold to 328.15 mg/L via the stepwise metabolic engineering of the original strain, including the overexpression of the rate-limiting genes (tHMG1 and ERG20) of the mevalonate pathway, transcription and protein level regulation of ERG9, Bts1p and Erg20
conclusionsIn this study, an efficient 13R-MO yeast cell factory was constructed, which achieved the de novo production of 3 g/L of 13R-MO from glucose. To the best of our knowledge, this is the highest 13R-MO titer reported to date. Furthermore, the metabolic engineering strategies presented here could be used to produce other valuable diterpenoid compounds in yeast.
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