ArticlePLoS neglected tropical diseases2019
The arginine sensing and transport binding sites are distinct in the human pathogen Leishmania.
Article in PLoS neglected tropical diseases, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Targeting Leishmania donovani Sphingosine Kinase 1 using PF-543 enhances immune response and limits parasite load.PLoS neglected tropical diseases · 2026Article
- Polyamine Metabolism inMedical sciences (Basel, Switzerland) · 2022Review
- New Vistas in the Biology of the Flagellum-Pathogens (Basel, Switzerland) · 2022Review
- Arginine and Arginases Modulate Metabolism, Tumor Microenvironment and Prostate Cancer Progression.Nutrients · 2021Review
- Nutrient sensing in Leishmania: Flagellum and cytosol.Molecular microbiology · 2021Review
- Arginase/nitric oxide modifications using live non-pathogenicJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2021Article
- Sensing Host Arginine Is Essential formBio · 2020Article
- Touching the Surface: Diverse Roles for the Flagellar Membrane in Kinetoplastid Parasites.Microbiology and molecular biology reviews : MMBR · 2020Review
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5 authors.
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Abstract
The intracellular protozoan parasite Leishmania donovani causes human visceral leishmaniasis. Intracellular L. donovani that proliferate inside macrophage phagolysosomes compete with the host for arginine, creating a situation that endangers parasite survival. Parasites have a sensor that upon arginine deficiency activates an Arginine Deprivation Response (ADR). L. donovani transport arginine via a high-affinity transporter (LdAAP3) that is rapidly up-regulated by ADR in intracellular amastigotes. To date, the sensor and its ligand have not been identified. Here, we show that the conserved amidino group at the distal cap of the arginine side chain is the ligand that activates ADR, in both promastigotes and intracellular amastigotes, and that arginine sensing and transport binding sites are distinct in L. donovani. Finally, upon addition of arginine and analogues to deprived cells, the amidino ligand activates rapid degradation of LdAAP3. This study provides the first identification of an intra-molecular ligand of a sensor that acts during infection.
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