ArticleMolecular biology of the cell2019
Microtubule bundling by MAP65-1 protects against severing by inhibiting the binding of katanin.
Article in Molecular biology of the cell, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 33 citations in OpenAlex.
- ROS inhibits microtubule dynamics and cell growth heterogeneity during Arabidopsis sepal morphogenesis.iScience · 2026Article
- N-terminal phosphorylation inhibits Arabidopsis katanin and affects vegetative and reproductive development in opposite ways.bioRxiv : the preprint server for biology · 2026Article
- Regulation of cytoskeleton dynamics and its interplay with force in plant cells.Biophysics reviews · 2024Review
- Interplay of self-organization of microtubule asters and crosslinking protein condensates.PNAS nexus · 2023Article
- Overlapping Localization of MAP65-2, -6, and -7 in Arabidopsis Hypocotyl Cells.microPublication biology · 2023Article
- Cell biology of primary cell wall synthesis in plants.The Plant cell · 2022Review
- Finding a right place to cut: How katanin is targeted to cellular severing sites.Quantitative plant biology · 2022Review
- Article
- Planar cell polarity induces local microtubule bundling for coordinated ciliary beating.The Journal of cell biology · 2021Article
- GR24, A Synthetic Strigolactone Analog, and Light Affect the Organization of Cortical Microtubules in Arabidopsis Hypocotyl Cells.Frontiers in plant science · 2021Article
- AtSEC22 Regulates Cell Morphogenesis via Affecting Cytoskeleton Organization and Stabilities.Frontiers in plant science · 2021Article
- TANGLED1 mediates microtubule interactions that may promote division plane positioning in maize.The Journal of cell biology · 2020Article
- Complementary Superresolution Visualization of Composite Plant Microtubule Organization and Dynamics.Frontiers in plant science · 2020Article
- Predicted Effects of Severing Enzymes on the Length Distribution and Total Mass of Microtubules.Biophysical journal · 2019Article
- Misplaced Golgi Elements Produce Randomly Oriented Microtubules and Aberrant Cortical Arrays of Microtubules in Dystrophic Skeletal Muscle Fibers.Frontiers in cell and developmental biology · 2019Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The microtubule-severing enzyme katanin (KTN1) regulates the organization and turnover of microtubule arrays by the localized breakdown of microtubule polymers. In land plants, KTN1 activity is essential for the formation of linearly organized cortical microtubule arrays that determine the axis of cell expansion. Cell biological studies have shown that even though KTN1 binds to the sidewalls of single and bundled microtubules, severing activity is restricted to microtubule cross-over and nucleation sites, indicating that cells contain protective mechanisms to prevent indiscriminate microtubule severing. Here, we show that the microtubule-bundling protein MAP65-1 inhibits KTN1-mediated microtubule severing in vitro. Severing is inhibited at bundled microtubule segments and the severing rate of nonbundled microtubules is reduced by MAP65-1 in a concentration-dependent manner. Using various MAP65-1 mutant proteins, we demonstrate that efficient cross-linking of microtubules is crucial for this protective effect and that microtubule binding alone is not sufficient. Reduced severing due to microtubule bundling by MAP65-1 correlated to decreased binding of KTN1 to these microtubules. Taken together, our work reveals that cross-linking of microtubules by MAP65-1 confers resistance to severing by inhibiting the binding of KTN1 and identifies the structural features of MAP65-1 that are important for this activity.
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