Evidence map›Paper›PMID 31017543›Full record

ArticleBioengineered2019

The pilot-scale preparation of the SA-hGM-CSF bi-functional fusion protein.

Xiaoqing Li, Shirong Zhou, Yao Wang, Hui Lian, Anxin Zuo, Kaihua Zhou, Ling Tong, Zhujun Zhou, Jimin Gao

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioengineered, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 97% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Xiaoqing Lia Zhejiang Provincial Key Lab for Technology & Application of Model Organisms,School of Laboratory Medicine and Life Sciences , Wenzhou Medical University , Wenzhou , China.
Shirong Zhoua Zhejiang Provincial Key Lab for Technology & Application of Model Organisms,School of Laboratory Medicine and Life Sciences , Wenzhou Medical University , Wenzhou , China.
Yao Wanga Zhejiang Provincial Key Lab for Technology & Application of Model Organisms,School of Laboratory Medicine and Life Sciences , Wenzhou Medical University , Wenzhou , China.
Hui Liana Zhejiang Provincial Key Lab for Technology & Application of Model Organisms,School of Laboratory Medicine and Life Sciences , Wenzhou Medical University , Wenzhou , China.
Anxin Zuoa Zhejiang Provincial Key Lab for Technology & Application of Model Organisms,School of Laboratory Medicine and Life Sciences , Wenzhou Medical University , Wenzhou , China.
Kaihua Zhoua Zhejiang Provincial Key Lab for Technology & Application of Model Organisms,School of Laboratory Medicine and Life Sciences , Wenzhou Medical University , Wenzhou , China.
Ling Tonga Zhejiang Provincial Key Lab for Technology & Application of Model Organisms,School of Laboratory Medicine and Life Sciences , Wenzhou Medical University , Wenzhou , China.
Zhujun Zhoua Zhejiang Provincial Key Lab for Technology & Application of Model Organisms,School of Laboratory Medicine and Life Sciences , Wenzhou Medical University , Wenzhou , China.
Jimin Gaoa Zhejiang Provincial Key Lab for Technology & Application of Model Organisms,School of Laboratory Medicine and Life Sciences , Wenzhou Medical University , Wenzhou , China.
Wenzhou Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The granulocyte-macrophage colony-stimulating factor (GM-CSF) can be used to induce a powerful immune response. Based on the specific binding of biotin and streptavidin, SA-hGM-CSF was anchored on the surface of biotinylated tumor cells, which could enhance the anti-tumor effect of tumor cell vaccines in our previous reports, suggesting it would have potential clinical value. Preparation of the biologically active proteins in large-scale production is the basis of clinical application, however, only a small amount of biologically active protein was obtained according to previous studies. In this study, we researched the effects of various factors on the purification and simultaneous renaturation of SA-hGM-CSF fusion protein by single factor experiment and orthogonal experiment. Here, we developed a viable pilot-scale trial in the fermentation, purification, refolding and freeze-drying of SA-hGM-CSF proteins in order to efficiently obtain more biologically active proteins with high purity, which will lay the foundation for industrial production.

Indexed as

Amino Acid SequenceAnimalsBiotinBiotinylationCell Line, TumorCloning, MolecularEscherichia coliFactor Analysis, StatisticalGene ExpressionGenetic VectorsGranulocyte-Macrophage Colony-Stimulating FactorHumansMicePC-3 CellsPilot ProjectsProtein DenaturationBiotinGranulocyte-Macrophage Colony-Stimulating FactorRecombinant Fusion ProteinsStreptavidinoptimizationpilot-scalepurificationrefoldingSA-hGM-CSF fusion protein

Identifiers

PMID31017543
PMCPMC6527079
OpenAlexW2942491033

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.