Evidence map›Paper›PMID 31017269›Full record

ArticleInternational journal of molecular medicine2019

Modulation of proliferation and differentiation of gingiva‑derived mesenchymal stem cells by concentrated growth factors: Potential implications in tissue engineering for dental regeneration and repair.

Xiao Chen, Yuhe Chen, Yali Hou, Peng Song, Minyue Zhou, Minhai Nie, Xuqian Liu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular medicine, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed.

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  14. [Effect of concentrated growth factor combined with mineralized collagen material on the adhesion, proliferation, and osteogenic differentiation of bone marrow mesenchymal stem cells and the osteogenic effectZhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Xiao ChenDepartment of Orthodontics, Mianyang Stomatological Hospital, Mianyang, Sichuan 621000, P.R. China.
Yuhe ChenLaboratory of Oral and Maxillofacial Reconstruction and Regeneration, Southwest Medical University, Luzhou, Sichuan 646000, P.R. China.
Yali HouDepartment of Oral Pathology, College and Hospital of Stomatology, Hebei Medical University, Shijiazhuang, Hebei 050000, P.R. China.
Peng SongDepartment of Oral Pathology, College and Hospital of Stomatology, Hebei Medical University, Shijiazhuang, Hebei 050000, P.R. China.
Minyue ZhouLaboratory of Oral and Maxillofacial Reconstruction and Regeneration, Southwest Medical University, Luzhou, Sichuan 646000, P.R. China.
Minhai NieLaboratory of Oral and Maxillofacial Reconstruction and Regeneration, Southwest Medical University, Luzhou, Sichuan 646000, P.R. China.
Xuqian LiuLaboratory of Oral and Maxillofacial Reconstruction and Regeneration, Southwest Medical University, Luzhou, Sichuan 646000, P.R. China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The aim of the present study was to evaluate the proliferation and osteogenic differentiation ability of gingiva‑derived mesenchymal stem cells (GMSCs) cultured with different concentrations of concentrated growth factors (CGF). GMSCs were isolated from gingival connective tissues and characterized by flow cytometry, immunofluorescence staining and immunohistochemical staining. Cell proliferation activity was determined by the MTT assay, and the effect of CGF on MCSCs was detected with the Cell Counting Kit (CCK)‑8 assay. Mineralization induction was evaluated by alkaline phosphatase (ALP)‑positive cell staining and mineralized nodule formation assay. Dentin matrix acidic phosphoprotein (DMP)1, dentin sialophosphoprotein (DSPP), bone morphogenetic protein (BMP)2 and runt‑related transcription factor (RUNX)2 mRNA and protein expression were evaluated by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) analysis and western blotting. The flow cytometry, immunofluorescence staining and immunohistochemical staining results indicated that the cultured cells were GMSCs. The MTT assay results revealed that the third‑generation gingival stem cells exhibited the highest proliferative capacity, and the CCK‑8 results indicated that 10% CGF achieved the most prominent promotion of GMSC proliferation. ALP activity analysis and mineralized nodule assay demonstrated that CGF may successfully induce osteogenic differentiation of GMSCs, whereas RT‑qPCR and western blot analyses demonstrated that CGF is involved in the differentiation of GMSCs by regulating the expression of DMP1, DSPP, BMP2 and RUNX2 (P<0.05). In conclusion, CGF were demonstrated to promote the proliferation and osteogenic differentiation of GMSCs. Therefore, CGF may be applied in tissue engineering for tooth regeneration and repair.

Indexed as

Tissue EngineeringCell DifferentiationCell ProliferationFemaleGingivaHumansIntercellular Signaling Peptides and ProteinsMaleMesenchymal Stem CellsRegenerationToothIntercellular Signaling Peptides and Proteins

Identifiers

PMID31017269
PMCPMC6559294

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.