Evidence map›Paper›PMID 31016701›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2019

Preparation and Purification of Oligodeoxynucleotide Duplexes Containing a Site-Specific, Reduced, Chemically Stable Covalent Interstrand Cross-Link Between a Guanine Residue and an Abasic Site.

Maryam Imani Nejad, Xu Guo, Kurt Housh, Christopher Nel, Zhiyu Yang, Nathan E Price, Yinsheng Wang, Kent S Gates

Abstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Article
  6. Review
  7. Article
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Maryam Imani NejadDepartment of Chemistry, University of Missouri, Columbia, MO, USA.
Xu GuoDepartment of Chemistry, University of Missouri, Columbia, MO, USA.
Kurt HoushDepartment of Chemistry, University of Missouri, Columbia, MO, USA.
Christopher NelDepartment of Chemistry, University of Missouri, Columbia, MO, USA.
Zhiyu YangDepartment of Chemistry, University of Missouri, Columbia, MO, USA.
Nathan E PriceDepartment of Chemistry, University of California Riverside, Riverside, CA, USA.
Yinsheng WangDepartment of Chemistry, University of California Riverside, Riverside, CA, USA.
Kent S GatesDepartment of Chemistry, University of Missouri, Columbia, MO, USA. gatesk@missouri.edu.

Funding

Cross-links At Abasic Sites in Duplex DNAR01ES021007 · NIEHS · UNIVERSITY OF MISSOURI-COLUMBIA · PI GATES, KENT S, WANG, YINSHENG · 2012 to 2023
$3.7M
NIEHS NIH HHS R01 ES021007
6 · The paper itself

Abstract

Methods for the preparation of DNA duplexes containing interstrand covalent cross-links may facilitate research in the fields of biochemistry, molecular biology, nanotechnology, and materials science. Here we report methods for the synthesis and isolation of DNA duplexes containing a site-specific, chemically stable, reduced covalent interstrand cross-link between a guanine residue and an abasic site. The method uses experimental techniques and equipment that are common in most biochemical laboratories and inexpensive, commercially available oligonucleotides and reagents.

Indexed as

Cross-Linking ReagentsGuanineNucleic Acid HeteroduplexesOligodeoxyribonucleotidesCross-Linking ReagentsGuanineNucleic Acid HeteroduplexesOligodeoxyribonucleotidesAbasic siteCross-link synthesisGel electrophoresisInterstrand DNA cross-linkPost-synthetic oligonucleotide modificationReductive amination

Identifiers

PMID31016701
PMCPMC8660138

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.