Evidence map›Paper›PMID 31016406›Full record

ArticleAMB Express2019

Real-time loop-mediated isothermal amplification: an early-warning tool for quarantine plant pathogen detection.

Chiara Aglietti, Nicola Luchi, Alessia Lucia Pepori, Paola Bartolini, Francesco Pecori, Aida Raio, Paolo Capretti, Alberto Santini

Open access · goldAbstract read
In one paragraph

Article in AMB Express, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
10.8field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 69 citations in OpenAlex.

  1. Ultrasensitive Detection ofBiosensors · 2026
    Article
  2. Development of an RT-LAMP Assay for DetectingDiagnostics (Basel, Switzerland) · 2025
    Article
  3. Review
  4. Review
  5. Article
  6. Detecting PathogenicMolecules (Basel, Switzerland) · 2024
    Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Review
  12. Article
  13. Rapid Detection ofPlants (Basel, Switzerland) · 2021
    Article
  14. Article
  15. Review
  16. Review
  17. Review
  18. Article
  19. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Chiara AgliettiInstitute for Sustainable Plant Protection, National Research Council (IPSP-CNR), Via Madonna del Piano 10, 50019, Sesto Fiorentino, Firenze, Italy.
Nicola LuchiInstitute for Sustainable Plant Protection, National Research Council (IPSP-CNR), Via Madonna del Piano 10, 50019, Sesto Fiorentino, Firenze, Italy. nicola.luchi@ipsp.cnr.it.ORCID http://orcid.org/0000-0003-3119-7574
Alessia Lucia PeporiInstitute for Sustainable Plant Protection, National Research Council (IPSP-CNR), Via Madonna del Piano 10, 50019, Sesto Fiorentino, Firenze, Italy.
Paola BartoliniInstitute for Sustainable Plant Protection, National Research Council (IPSP-CNR), Via Madonna del Piano 10, 50019, Sesto Fiorentino, Firenze, Italy.
Francesco PecoriInstitute for Sustainable Plant Protection, National Research Council (IPSP-CNR), Via Madonna del Piano 10, 50019, Sesto Fiorentino, Firenze, Italy.
Aida RaioInstitute for Sustainable Plant Protection, National Research Council (IPSP-CNR), Via Madonna del Piano 10, 50019, Sesto Fiorentino, Firenze, Italy.
Paolo CaprettiDepartment of Agrifood Production and Environmental Sciences (DISPAA), University of Florence, Piazzale delle Cascine 28, 50144, Firenze, Italy.
Alberto SantiniInstitute for Sustainable Plant Protection, National Research Council (IPSP-CNR), Via Madonna del Piano 10, 50019, Sesto Fiorentino, Firenze, Italy.
Institute for Sustainable Plant Protection · ITUniversity of Florence · IT

Funding

Fondazione Cassa di Risparmio di Pistoia e Pescia 255Horizon 2020 research and innovation programme 771271
6 · The paper itself

Abstract

An effective framework for early warning and rapid response is a crucial element to prevent or mitigate the impact of biological invasions of plant pathogens, especially at ports of entry. Molecular detection of pathogens by using PCR-based methods usually requires a well-equipped laboratory. Rapid detection tools that can be applied as point-of-care diagnostics are highly desirable, especially to intercept quarantine plant pathogens such as Xylella fastidiosa, Ceratocystis platani and Phytophthora ramorum, three of the most devastating pathogens of trees and ornamental plants in Europe and North America. To this aim, in this study we developed three different loop mediated isothermal amplification (LAMP) assays able to detect each target pathogen both in DNA extracted from axenic culture and in infected plant tissues. By using the portable instrument Genie

Indexed as

Alien pathogensCanker Stain DiseaseIsothermal amplificationLAMPOlive Quick Decline SyndromePortable diagnosticsSudden Oak Death

Identifiers

PMID31016406
PMCPMC6478783
OpenAlexW2945663227

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.