ArticleJournal of biological engineering2019
A simple approach for rapid and cost-effective quantification of extracellular vesicles using a fluorescence polarization technique.
Article in Journal of biological engineering, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 27 citations in OpenAlex.
- Recent advances in aptamer-based biosensing technology for isolation and detection of extracellular vesicles.Frontiers in cell and developmental biology · 2025Review
- An aptamer-guided fluorescence polarisation platform for extracellular vesicle liquid biopsy.Journal of extracellular vesicles · 2024Article
- Exploring salivary exosomes as early predictors of oral cancer in susceptible tobacco consumers: noninvasive diagnostic and prognostic applications.Journal of cancer research and clinical oncology · 2023Article
- Extracellular Vesicles, as Drug-Delivery Vehicles, Improve the Biological Activities of Astaxanthin.Antioxidants (Basel, Switzerland) · 2023Article
- Recent developments in biosensing methods for extracellular vesicle protein characterization.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2023Review
- Proof of concept of using a membrane-sensing peptide for sEVs affinity-based isolation.Frontiers in bioengineering and biotechnology · 2023Article
- Graphene Oxide Enhances Biogenesis and Release of Exosomes in Human Ovarian Cancer Cells.International journal of nanomedicine · 2022Article
- Article
- Immunoassay-aptasensor for the determination of tumor-derived exosomes based on the combination of magnetic nanoparticles and hybridization chain reaction.RSC advances · 2021Article
- Palladium Nanoparticle-Induced Oxidative Stress, Endoplasmic Reticulum Stress, Apoptosis, and Immunomodulation Enhance the Biogenesis and Release of Exosome in Human Leukemia Monocytic Cells (THP-1).International journal of nanomedicine · 2021Article
- Platinum Nanoparticles Enhance Exosome Release in Human Lung Epithelial Adenocarcinoma Cancer Cells (A549): Oxidative Stress and the Ceramide Pathway are Key Players.International journal of nanomedicine · 2021Article
- Amphipathic helical peptide-based fluorogenic probes for a marker-free analysis of exosomes based on membrane-curvature sensing.RSC advances · 2020Article
- Immunoassay-type biosensor based on magnetic nanoparticle capture and the fluorescence signal formed by horseradish peroxidase catalysis for tumor-related exosome determination.Mikrochimica acta · 2020Article
- Signature mRNA markers in extracellular vesicles for the accurate diagnosis of colorectal cancer.Journal of biological engineering · 2020Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Extracellular vesicles (EVs) are membrane-bound phospholipid vesicles actively secreted by all cells. As they carry specific markers expressed by their parental cells, EVs are utilized to identify specific cells via liquid biopsy. To facilitate EV-based clinical diagnosis, a fast and reliable method to count EVs is critical. We developed a method for rapid and cost-effective quantification of EVs which relies on the fluorescence polarization (FP) detection of lipophilic fluorescein probe, 5-dodecanoylamino fluorescein (C12-FAM). The alkyl tail of C12-FAM is specifically incorporated into the EVs, producing high FP values due to a slow diffusional motion. We quantified EVs derived from two cell lines, HT29 and TCMK1 using the new strategy, with good sensitivity that was at par with the commercial method. The new method involves minimal complexity and hands-on time. In addition, FP signaling is inherently ratiometric and is robust against environmental noise.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.