Evidence map›Paper›PMID 31015791›Full record

ArticleCurrent genomics2019

Integrated Transcriptome Analysis of microRNA and mRNA in Mouse Skin Derived Precursors (SKPs) and SKP Derived Fibroblast (SFBs) by RNA-Seq.

Rongying Zhou, Yujie Mao, Lidan Xiong, Li Li

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Current genomics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Rongying Zhou1Department of Dermatology, West China Hospital, Sichuan University, Chengdu610041, China; 2Department of Dermatology, Sichuan Academy of Science & Sichuan Provincial People's Hospital, University of Electronic Science and Technology, Chengdu610072, China.
Yujie Mao1Department of Dermatology, West China Hospital, Sichuan University, Chengdu610041, China; 2Department of Dermatology, Sichuan Academy of Science & Sichuan Provincial People's Hospital, University of Electronic Science and Technology, Chengdu610072, China.
Lidan Xiong1Department of Dermatology, West China Hospital, Sichuan University, Chengdu610041, China; 2Department of Dermatology, Sichuan Academy of Science & Sichuan Provincial People's Hospital, University of Electronic Science and Technology, Chengdu610072, China.
Li Li1Department of Dermatology, West China Hospital, Sichuan University, Chengdu610041, China; 2Department of Dermatology, Sichuan Academy of Science & Sichuan Provincial People's Hospital, University of Electronic Science and Technology, Chengdu610072, China.
Sichuan University · CNSichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundSkin-derived precursors (SKPs) display the characteristics of self-renewal and multilineage differentiation.

objectiveThe study aimed to explore the molecular mechanisms of mouse SKPs differentiation into SKP-derived fibroblasts (SFBs).

methodsWe compared the microRNA (miRNA) profile in mouse SKPs and SFBs by RNA sequenc-ing. Real-time quantitative reverse transcription PCR (qRT-PCR) was performed to validate the miRNA expression. The integrated analysis of miRNA and mRNA expression data was performed to explore the potential crosstalk of miRNA-mRNA in SKP differentiation.

results207 differentially expressed miRNAs and 835 miRNA target genes in the gene list of integrated mRNA expression profiling were identified. Gene Ontology (GO) enrichment analysis revealed that cell differentiation and cell proliferation process were significantly enriched. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed the target genes were significantly most enriched in the cytokine-cytokine receptor interaction, cancer pathways and axon guidance signaling pathway. The most upregulated and downregulated target genes were involved in the Wnt, Notch, cytokine-cytokine receptor interaction, TGF-β, p53 and apoptotic signaling pathway. The miRNA-mRNA regulatory net-works and 507 miRNA-mRNA pairs were constructed. Seven miRNAs (miR-486-3p, miR-504-5p, miR-149-3p, miR-31-5p, miR-484, miR-328-5p and miR-22-5p) and their target genes Wnt4, Dlx2, Se-ma4f, Kit, Kitl, Inpp5d, Igfbp3, Prdm16, Sfn, Irf6 and Clu were identified as miRNA-mRNA crosstalk pairs.

conclusionThese genes and signaling pathways might control SKPs proliferation and SKPs differen-tiation into SFBs during the process of SKPs differentiation, which might promote the application of SKPs in the clinical treatment of skin-related diseases by regulating SKPs proliferation and SKPs differ-entiation.

Indexed as

FibroblastsmicroRNARNA sequencingSkin derived precursorsStem cellTranscriptome analysis

Identifiers

PMID31015791
PMCPMC6446482
OpenAlexW2895889600

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.