ArticleScientific reports2019
User-defined morphogen patterning for directing human cell fate stratification.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed.
- Optogenetic BMP4 models symmetry breaking and primitive streak formation in hPSCs.bioRxiv : the preprint server for biology · 2026Article
- Topological Tracks Patterned via 3D Printing Vascularize Murine Organ-Scale Constructs.Cell biomaterials · 2026Article
- Bioengineering innovations for neural organoids with enhanced fidelity and function.Cell stem cell · 2025Review
- A microfluidic platform for anterior-posterior human endoderm patterning via countervailing morphogen gradientsiScience · 2025Article
- Microgels With Electrostatically Controlled Molecular Affinity to Direct Morphogenesis.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- Modeling development using hydrogels.Development (Cambridge, England) · 2023Article
- Microfluidic systems for modeling human development.Development (Cambridge, England) · 2022Article
- Engineering Heart Morphogenesis.Trends in biotechnology · 2020Review
- Recent Advancements in Engineering Strategies for Manipulating Neural Stem Cell Behavior.Current tissue microenvironment reports · 2020Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Concentration gradients of biochemical stimuli such as morphogens play a critical role in directing cell fate patterning across species and throughout development but are not commonly recapitulated in vitro. While in vitro biomolecule gradients have been generated using customized microfluidic platforms, broad implementation has been limited because these platforms introduce new variables to cell culture such as externally driven flow, culture in a specialized matrix, or extended time for in situ long range diffusion. Here we introduce a method that enables preforming and then transferring user-controlled gradients to cells in standard "open" cultures. Our gradient patterning devices are modular and decoupled from the culture substrate. We find that gradient generation and transfer are predictable by finite element modeling and that device and loading parameters can be used to tune the stimulus pattern. Furthermore, we demonstrate use of these devices to spatially define morphogen signal gradients and direct peri-gastrulation fate stratification of human pluripotent stem cells. This method for extrinsic application of biochemical signal gradients can thus be used to spatially influence cellular fate decisions in a user-controlled manner.
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Registered trials
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