ArticleBMC genomics2019
Comprehensive characterization of T-DNA integration induced chromosomal rearrangement in a birch T-DNA mutant.
Article in BMC genomics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 54 citations in OpenAlex.
- Unintended effects of exogenous DNA integration in transgenic papaya analyzed at DNA and transcriptional levels.BMC plant biology · 2026Article
- Beyond a few bases: methods for large DNA insertion and gene targeting in plants.The Plant journal : for cell and molecular biology · 2025Review
- Transgenic poplar (Populus davidiana×P. bolleana Loucne) expressing dsRNA of insect chitinase gene: lines identification and resistance assay.Journal of insect science (Online) · 2024Article
- Genomic consequences associated with Agrobacterium-mediated transformation of plants.The Plant journal : for cell and molecular biology · 2024Review
- Microbiome and plant cell transformation trigger insect gall induction in cassava.Frontiers in plant science · 2023Article
- The Role of Carbonic Anhydrase αCA4 in Photosynthetic Reactions inPlants (Basel, Switzerland) · 2022Article
- CRISPR/Cas9-Mediated Targeted DNA Integration: Rearrangements at the Junction of Plant and Plasmid DNA.International journal of molecular sciences · 2022Article
- Gene targeting in polymerase theta-deficient Arabidopsis thaliana.The Plant journal : for cell and molecular biology · 2022Article
- Large scale genomic rearrangements in selected Arabidopsis thaliana T-DNA lines are caused by T-DNA insertion mutagenesis.BMC genomics · 2021Article
- Whole-genome resequencing using next-generation and Nanopore sequencing for molecular characterization of T-DNA integration in transgenic poplar 741.BMC genomics · 2021Article
- Genomic Variation Prediction: A Summary From Different Views.Frontiers in cell and developmental biology · 2021Review
- Physiological and Molecular Mechanism Involved in Cold Stress Tolerance in Plants.Plants (Basel, Switzerland) · 2020Review
- The Position and Complex Genomic Architecture of Plant T-DNA Insertions Revealed by 4SEE.International journal of molecular sciences · 2020Article
- Generation ofMicroorganisms · 2019Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
backgroundIntegration of T-DNA into plant genomes via Agrobacterium may interrupt gene structure and generate numerous mutants. The T-DNA caused mutants are valuable materials for understanding T-DNA integration model in plant research. T-DNA integration in plants is complex and still largely unknown. In this work, we reported that multiple T-DNA fragments caused chromosomal translocation and deletion in a birch (Betula platyphylla × B. pendula) T-DNA mutant yl.
resultsWe performed PacBio genome resequencing for yl and the result revealed that two ends of a T-DNA can be integrated into plant genome independently because the two ends can be linked to different chromosomes and cause chromosomal translocation. We also found that these T-DNA were connected into tandem fragment regardless of direction before integrating into plant genome. In addition, the integration of T-DNA in yl genome also caused several chromosomal fragments deletion. We then summarized three cases for T-DNA integration model in the yl genome. (1) A T-DNA fragment is linked to the two ends of a double-stranded break (DSB); (2) Only one end of a T-DNA fragment is linked to a DSB; (3) A T-DNA fragment is linked to the ends of different DSBs. All the observations in the yl genome supported the DSB repair model.
conclusionsIn this study, we showed a comprehensive genome analysis of a T-DNA mutant and provide a new insight into T-DNA integration in plants. These findings would be helpful for the analysis of T-DNA mutants with special phenotypes.
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