Evidence map›Paper›PMID 31014236›Full record

ArticleBMC genomics2019

Identification of genomic regions associated with multi-silique trait in Brassica napus.

Liang Chai, Jinfang Zhang, Kun Lu, Haojie Li, Lintao Wu, Hongshen Wan, Benchuan Zheng, Cheng Cui, Jun Jiang, Liangcai Jiang

Open access · goldAbstract read
In one paragraph

Article in BMC genomics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
0.9field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 17 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Liang ChaiCrop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, Sichuan, China.
Jinfang ZhangCrop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, Sichuan, China.
Kun LuCollege of Agronomy and Biotechnology, Southwest University, Chongqing, 400716, Beibei, China.
Haojie LiCrop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, Sichuan, China.
Lintao WuRape Research Institute, Guizhou Academy of Agricultural Sciences, Guiyang, 550008, China.
Hongshen WanCrop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, Sichuan, China.
Benchuan ZhengCrop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, Sichuan, China.
Cheng CuiCrop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, Sichuan, China.
Jun JiangCrop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, Sichuan, China.
Liangcai JiangCrop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, Sichuan, China. jlcrape@163.com.
Sichuan Academy of Agricultural Sciences · CNGuizhou Academy of Agricultural Sciences · CNSouthwest University · CN

Funding

Financial Innovation Ability Promotion Project of Sichuan Province 2016ZYPZ-013Modern Agro-industry Technology Research System of China CARS-12National Natural Science Foundation of China 31560402Scientific Observing and Experimental Station of Oil Crops in the Upper Yangtze River, Ministry of Agriculture, P. R. China 09203020The National Key Research and Development Plan 2018YFD0100500The National Key Research and Development Plan JFYS2016ZY03002156
6 · The paper itself

Abstract

backgroundAlthough rapeseed (Brassica napus L.) mutant forming multiple siliques was morphologically described and considered to increase the silique number per plant, an important agronomic trait in this crop, the molecular mechanism underlying this beneficial trait remains unclear. Here, we combined bulked-segregant analysis (BSA) and whole genome re-sequencing (WGR) to map the genomic regions responsible for the multi-silique trait using two pools of DNA from the near-isogenic lines (NILs) zws-ms (multi-silique) and zws-217 (single-silique). We used the Euclidean Distance (ED) to identify genomic regions associated with this trait based on both SNPs and InDels. We also conducted transcriptome sequencing to identify differentially expressed genes (DEGs) between zws-ms and zws-217.

resultsGenetic analysis using the ED algorithm identified three SNP- and two InDel-associated regions for the multi-silique trait. Two highly overlapped parts of the SNP- and InDel-associated regions were identified as important intersecting regions, which are located on chromosomes A09 and C08, respectively, including 2044 genes in 10.20-MB length totally. Transcriptome sequencing revealed 129 DEGs between zws-ms and zws-217 in buds, including 39 DEGs located in the two abovementioned associated regions. We identified candidate genes involved in multi-silique formation in rapeseed based on the results of functional annotation.

conclusionsThis study identified the genomic regions and candidate genes related to the multi-silique trait in rapeseed.

Indexed as

GenomicsBrassica napusGene Expression ProfilingINDEL MutationMolecular Sequence AnnotationPolymorphism, Single NucleotideQuantitative Trait LociSequence Homology, Nucleic AcidAssociation analysisBrassica napus L.Multi-siliqueNear-isogenic lineTranscriptome sequencingWhole genome re-sequencing

Identifiers

PMID31014236
PMCPMC6480887
OpenAlexW2947335431

What OpenQuestion holds

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LicenceCC BY
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.