ArticleOpen biology2019
Abnormal arachidonic acid metabolic network may reduce sperm motility via P38 MAPK.
Article in Open biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 47 citations in OpenAlex.
- Seminal Plasma Exosomal miRNA Profiling Reveals hsa-miR-7-5p as a Key Regulator of Sperm Motility in Asthenozoospermia.Andrology · 2026Article
- Age-Associated Metabolomic Changes in Human Spermatozoa.International journal of molecular sciences · 2026Article
- Seminal Plasma Metabolomic Profiling Reveals Key Metabolic Signatures Linked to Spermatogenic Potential in Non-Obstructive Azoospermia with Cryptorchidism.Metabolites · 2026Article
- Deciphering the Therapeutic Mechanisms of Wuzi Yanzong Pill for Asthenozoospermia: A Synergistic Approach Combining Bioinformatics and Molecular Dynamics.Cell biochemistry and biophysics · 2025Article
- GC-MS/MS analysis of seminal plasma PUFAs in distinct subgroups of infertile men: diagnostic potential and insight into mechanisms of male infertility.Scientific reports · 2025Observational
- D-Mannose-Mediated metabolic pathways sustain the molecular signatures of sperm function and fertilization.Journal of advanced research · 2025Article
- Oxidative Stress Induces Changes in Molecular Markers Associated with Ferroptosis in Human Spermatozoa.The world journal of men's health · 2025Article
- Assessment of Electroacupuncture Therapy with Distant-Approximal Acupoints Based on the HPT Axis in Rats with Oligoasthenospermia Through Transcriptomic Analysis.Reproductive sciences (Thousand Oaks, Calif.) · 2025Article
- Multi-omics analysis and experimental verification reveal testicular fatty acid metabolism disorder in non-obstructive azoospermia.Zoological research · 2025Article
- Revisiting the Injury Mechanism of Goat Sperm Caused by the Cryopreservation Process from a Perspective of Sperm Metabolite Profiles.International journal of molecular sciences · 2024Article
- Multiple transcriptome analyses reveal mouse testis developmental dynamics.BMC genomics · 2024Article
- Article
- Metabonomic analysis of seminal plasma in necrozoospermia patients based on liquid chromatography-mass spectrometry.Translational andrology and urology · 2023Article
- Regulation of semen quality by fatty acids in diets, extender, and semen.Frontiers in veterinary science · 2023Review
- Post-thaw quality of ram sperm frozen with different concentrations of low-density lipoproteins associated with non-enzymatic antioxidants.Animal reproduction · 2023Article
- Effect of Wuzi Yanzong prescription on oligoasthenozoospermia rats based on UPLC-Q-TOF-MS metabolomics.Pharmaceutical biology · 2022Article
- Metabolomics of Human Semen: A Review of Different Analytical Methods to Unravel Biomarkers for Male Fertility Disorders.International journal of molecular sciences · 2022Review
- Role of p38 MAPK Signalling in Testis Development and Male Fertility.Oxidative medicine and cellular longevity · 2022Review
- Male Infertility Knowledgebase: decoding the genetic and disease landscape.Database : the journal of biological databases and curation · 2021Article
- Nanomaterials and hepatic disease: toxicokinetics, disease types, intrinsic mechanisms, liver susceptibility, and influencing factors.Journal of nanobiotechnology · 2021Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Asthenozoospermia is a common cause of male infertility, the aetiology of which remains unclear in 50-60% of cases. The current study aimed to characterize metabolic alterations in asthenozoospermic seminal plasma and to explore the signalling pathways involved in sperm motility regulation. At first, high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry was used to detect the targeted metabolic network of arachidonic acid (AA). Metabolomic multivariate data analysis showed significant distinction of AA metabolites between asthenozoospermic and healthy seminal plasma. AA as well as its lipoxygenase (LOX) and cytochrome P450 metabolites were found to be abnormally increased, while cyclooxygenase (COX) metabolites were complicatedly disturbed in asthenozoospermic volunteers compared with those in healthy ones. In vitro experiments and western blot analysis of sperm cells revealed a decrease in sperm motility and upregulation of sperm phosphor-P38 induced by AA. P38 inhibitor could increase AA-reduced sperm motility. Also, all the inhibitors of the three metabolic pathways of AA could block AA-induced P38 mitogen-activated protein kinase (MAPK) activation and further improve sperm motility. We report here for the first time that an abnormal AA metabolic network could reduce sperm motility via P38 MAPK activation through the LOX, cytochrome P450 and COX metabolic pathways, which might be an underlying pathomechanism of asthenozoospermia.
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