ArticleJournal of cellular physiology2019
Spatial control of oxygen delivery to three-dimensional cultures alters cancer cell growth and gene expression.
Article in Journal of cellular physiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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Who cites it
12 citing papers in PubMed, 17 citations in OpenAlex.
- Harnessing human tumor organoids for cancer modeling and precision therapy.Protein & cell · 2026Review
- Preparation of Highly Functional Spheroid of Endocrine Cells Based on Thermosensitive Glycol Chitosan.Tissue engineering and regenerative medicine · 2025Article
- Engineered 3D ex vivo models to recapitulate the complex stromal and immune interactions within the tumor microenvironment.Biomaterials · 2024Review
- Comparison of Engineered Liver 3D Models and the Role of Oxygenation for Patient-Derived Tumor Cells and Immortalized Cell Lines Cocultured with Tumor Stroma in the Detection of Hepatotoxins.Advanced biology · 2024Article
- Development and characterization of a recombinant silk network for 3D culture of immortalized and fresh tumor-derived breast cancer cells.Bioengineering & translational medicine · 2023Article
- Design considerations of benchtop fluid flow bioreactors for bio-engineered tissue equivalentsBiomaterials and biosystems · 2022Article
- High-Throughput Dispensing of Viscous Solutions for Biomedical Applications.Micromachines · 2022Article
- Harnessing preclinical models for the interrogation of ovarian cancer.Journal of experimental & clinical cancer research : CR · 2022Review
- Setup of human liver-chips integrating 3D models, microwells and a standardized microfluidic platform as proof-of-concept study to support drug evaluation.Biomaterials and biosystems · 2022Article
- Metabolic Flexibility Is a Determinant of Breast Cancer Heterogeneity and Progression.Cancers · 2021Review
- Application of Ovarian Cancer Organoids in Precision Medicine: Key Challenges and Current Opportunities.Frontiers in cell and developmental biology · 2021Review
- Spatial control of oxygen delivery to three-dimensional cultures alters cancer cell growth and gene expression.Journal of cellular physiology · 2019Article
Corrections and comments
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Authors and funding
14 authors at 1 institution in 1 country.
Funding
Abstract
Commonly used monolayer cancer cell cultures fail to provide a physiologically relevant environment in terms of oxygen delivery. Here, we describe a three-dimensional (3D) bioreactor system where cancer cells are grown in Matrigel in modified six-well plates. Oxygen is delivered to the cultures through a polydimethylsiloxane (PDMS) membrane at the bottom of the wells, with microfabricated PDMS pillars to control oxygen delivery. The plates receive 3% oxygen from below and 0% oxygen at the top surface of the media, providing a gradient of 3-0% oxygen. We compared growth and transcriptional profiles for cancer cells grown in Matrigel in the bioreactor, 3D cultures grown in 21% oxygen, and cells grown in a standard hypoxia chamber at 3% oxygen. Additionally, we compared gene expression of conventional two-dimensional monolayer culture and 3D Matrigel culture in 21% oxygen. We conclude that controlled oxygen delivery may provide a more physiologically relevant 3D system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.