Evidence map›Paper›PMID 31012103›Full record

ArticleJournal of microscopy2019

A simple empirical algorithm for optimising depletion power and resolution for dye and system specific STED imaging.

Christian A Combs, Dan L Sackett, Jay R Knutson

Open access · greenAbstract read
In one paragraph

Article in Journal of microscopy, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.8field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Christian A CombsNHLBI Light Microscopy Facility, National Institutes of Health, Bethesda, Maryland, U.S.A.ORCID 0000-0003-4656-4840
Dan L SackettNICHD Division of Basic and Translational Biophysics, National Institutes of Health, Bethesda, Maryland, U.S.A.ORCID 0000-0002-3492-1866
Jay R KnutsonNHLBI Laboratory for Advanced Microscopy and Biophotonics, National Institutes of Health, Bethesda, Maryland, U.S.A.ORCID 0000-0001-9990-1738
National Institutes of Health · US

Funding

Light Microscopy CoreZICHL006019 · NHLBI · NATIONAL HEART, LUNG, AND BLOOD INSTITUTE · PI COMBS, CHRISTIAN · 2009 to 2025
$29.3M
Multiphoton Microscopy DevelopmentZIAHL006065 · NHLBI · NATIONAL HEART, LUNG, AND BLOOD INSTITUTE · PI KNUTSON, JAY R · 2010 to 2025
$11.4M
Microtubule regulation by small molecules.ZIAHD008765 · NICHD · EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT · PI SACKETT, DAN L · 2009 to 2025
$8.9M
Optical Superresolution Microscopy (Nanoscopy)ZIAHL006066 · NHLBI · NATIONAL HEART, LUNG, AND BLOOD INSTITUTE · PI KNUTSON, JAY R · 2010 to 2025
$5.3M
Time Resolved Fluorescence SpectroscopyZIAHL001452 · NHLBI · NATIONAL HEART, LUNG, AND BLOOD INSTITUTE · PI KNUTSON, JAY R · 2009 to 2025
$5.1M
Intramural NIH HHS Z99 HL999999Intramural NIH HHS ZIC HL006019
6 · The paper itself

Abstract

Here we show an easy method for determining an effective dye saturation factor ('P

Indexed as

AlgorithmsColoring AgentsCell Line, TumorHumansImage Processing, Computer-AssistedMicroscopy, FluorescenceMicrotubulesColoring AgentsSTED microscopysuper-resolution

Identifiers

PMID31012103
PMCPMC6553495
OpenAlexW2940589318

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.