ArticleResearch and practice in thrombosis and haemostasis2019
Shear stress and platelet-induced tensile forces regulate ADAMTS13-localization within the platelet thrombus.
Article in Research and practice in thrombosis and haemostasis, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 12 citations in OpenAlex.
- On a quest for fingerprints of crystalglobulinemia on platelets and endothelium.Blood vessels, thrombosis & hemostasis · 2026Article
- Impact of rise and fall phases of shear on platelet activation and aggregation using microfluidics.Journal of thrombosis and thrombolysis · 2024Article
- Association between ADAMTS13 deficiency and cardiovascular events in chronic hemodialysis patients.Scientific reports · 2021Observational
- Biomechanical thrombosis: the dark side of force and dawn of mechano-medicine.Stroke and vascular neurology · 2020Review
- Shear stress and platelet-induced tensile forces regulate ADAMTS13-localization within the platelet thrombus.Research and practice in thrombosis and haemostasis · 2019Article
- von Willebrand Factor as a Predictor for Transplant-Associated Thrombotic Microangiopathy.Clinical and applied thrombosis/hemostasis : official journal of the International Academy of Clinical and Applied Thrombosis/HemostasisArticle
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9 authors at 1 institution in 1 country.
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Abstract
backgroundThe multimeric glycoprotein von Willebrand factor (VWF) mediates platelet adhesion and aggregation at the site of vessel injury. The adhesive activity of VWF is influenced by its multimer length which is regulated by the metalloprotease ADAMTS13. The ability of ADAMTS13 to regulate platelet thrombus growth in a shear-dependent manner has been described, however, the mechanistic basis of this action has not been well characterized.
methodsWe developed an mCherry-tagged murine ADAMTS13 protein and utilized an ex vivo flow chamber system to visualize the localization of ADAMTS13 within the platelet thrombus under different conditions of shear. Using this system, we also assessed the influence of platelet-mediated tensile force on ADAMTS13 localization within the thrombus using gain-of-function GPIb binding and loss-of-function GPIIbIIIa binding mutants in VWF/ADAMTS13 DKO mice.
resultsADAMTS13 was visualized on the growing platelet thrombus under very high shear using ADAMTS13-mcherry. ADAMTS13-mCherry localized particularly at the top portion of the thrombus and reduced thrombus size as it grew to occlusion. At the pathological high shear of 7500 s
conclusionsTensile force applied on VWF produced by shear stress and platelet GPIb binding has a crucial role in ADAMTS13 activity at the site of thrombus formation. These results suggest that ADAMTS13 activity at the site of platelet thrombus formation is regulated by a shear stress and platelet-dependent feedback mechanism to prevent vessel occlusion and pathological thrombosis.
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