ArticleLab animal2019
Experiments done in Black-6 mice: what does it mean?
Article in Lab animal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed.
- Considerations for the selection and phenotyping of mouse models for the study of Alzheimer's disease.STAR protocols · 2026Review
- DSS Colitis Model: Traps, Tricks, and Reporting Recommendations.Biomedicines · 2026Review
- The rise and deceleration of neuronal excitability in aging and Alzheimer's disease: Mechanisms, implications, and therapeutic targets.Ageing research reviews · 2026Review
- Substrain-specific behavioral variation in female C57BL/6 and C57BL/10 mice.Frontiers in behavioral neuroscience · 2026Article
- Inactive but awake behaviour as indicating a depression-like state in mice: aetiological factors and association with adult hippocampal neurogenesis.Royal Society open science · 2025Article
- Brief introduction in phenotypic and genetic differences of C57BL/6 and BALB/c mice substrains.Animal models and experimental medicine · 2025Review
- Comparative phenotyping of C57BL/6J substrains reveals distinctive patterns of cardiac aging.GeroScience · 2025Article
- Effect of Mouse (Mus musculus) Sex and C57BL/6 Substrain on Sensitivity to Isoflurane and Ketamine-Xylazine-Acepromazine Anesthesia.Journal of the American Association for Laboratory Animal Science : JAALAS · 2025Article
- Unveiling the Phenotypic Variability of Macrophages: Insights from Donor Diversity and Pooling Strategies.International journal of molecular sciences · 2025Article
- Deficiency of GPR10 and NPFFR2 receptors leads to sex-specific prediabetic syndrome and late-onset obesity in mice.Bioscience reports · 2024Article
- Nontraditional models as research tools: the road not taken.Trends in molecular medicine · 2024Review
- Microenvironment M1/M2 macrophages and tumoral progression vary within C57BL/6 mice from same substrain in prostate cancer model.Scientific reports · 2024Article
- A practical guide for the husbandry of cave and surface invertebrates as the first step in establishing new model organisms.PloS one · 2024Article
- Cyfip2 allelic variation in C57BL/6J and C57BL/6NJ mice alters free-choice ethanol drinking but not binge-like drinking or wheel-running activity.Alcohol, clinical & experimental research · 2023Article
- Delay eyeblink conditioning performance and brain-wide c-Fos expression in male and female mice.Open biology · 2023Article
- Substrain differences in Sry expression at the stage of sex determination in C57BL/6 mouse strains.The Journal of veterinary medical science · 2023Article
- How stra(i)nge are your controls? A comparative analysis of metabolic phenotypes in commonly used C57BL/6 substrains.PloS one · 2023Article
- Norecopa: A global knowledge base of resources for improving animal research and testing.Frontiers in veterinary science · 2023Article
- Genetic background determines synaptic phenotypes inFrontiers in psychiatry · 2023Article
- Social deficits in BTBR T+ Itpr3tf/J mice vary with ecological validity of the test.Genes, brain, and behavior · 2022Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Low replicability of animal experiments is perceived as a major hurdle in the field of biomedicine. Attempts to enhance the replicability and to reduce the variability in basic research has led to the recommendation to use isogenic mice. The C57BL/6 strain has evolved as a gold standard strain for this purpose. However, C57BL/6 mice are maintained as substrains by multiple vendors. Evidence exists that the subtle differences between these mouse lines have not been systematically investigated and are often ignored. In the present study, we characterized the female mice of two closely related substrains (C57BL/6J and C57BL/6N) from three vendors in Europe (Charles River Laboratories, Envigo, Janvier Labs) in a battery of behavioral tests. Our data show and confirm substantial behavioral differences between the C57BL/6J and C57BL/6N mice. Importantly, the substrain differences were largely affected by the origin of the animals, as a significant effect of vendor or interaction between the substrain and vendor occurred in all tests. This work highlights the importance of adhering to precise international nomenclature in all publications reporting animal experiments. Moreover, the generalization of research findings from a single mouse substrain can be seriously limited due to genetic drift and environmental variables occurring at different vendors. However, heterogenization of samples, by including animals of different substrains, can enhance generalizability. These issues need to be seriously addressed to improve reproducibility, replicability, and the translational potential of the mouse models.
Indexed as
Identifiers
31011223What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.