ArticleNature biotechnology2019
Increasing the accuracy of nanopore DNA sequencing using a time-varying cross membrane voltage.
Article in Nature biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed.
- HELQ & Hel308: ancient enzymes of DNA repair and recombination.Biochemical Society transactions · 2026Review
- Long-read sequencing technologies and bioinformatics: a new perspective for decoding DNA methylation modifications.Journal of translational medicine · 2026Review
- Application of nanopore adaptive sampling for metagenomic detection of tick-borne RNA viruses.Scientific reports · 2026Article
- An information theory approach to quantifying the sequence-dependent response of nucleic acid motors with applications to nanopore DNA sequencing.Nature communications · 2026Article
- Heterogeneous Nanopore Arrays - Selective Modification of Nanopores Embedded in a Membrane.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- Leveraging illumina iSeq100 for whole genome sequencing of Salmonella Typhi for a resource-limited setting.Scientific reports · 2025Article
- Sequencing a DNA analog composed of artificial bases.Nature communications · 2025Article
- Direct high-throughput deconvolution of non-canonical bases via nanopore sequencing and bootstrapped learning.Nature communications · 2025Article
- NANOME: A Nextflow pipeline for haplotype-aware allele-specific consensus DNA methylation detection by nanopore long-read sequencing.bioRxiv : the preprint server for biology · 2025Article
- Eliminating the Interference of Neighboring Nucleobases in Aerolysin for Nanopore Sequencing.ACS sensors · 2025Article
- Redesign of Translocon EXP2 Nanopore for Detecting Peptide Fragments.Small methods · 2025Article
- Nanopore sequencing in veterinary medicine: from concepts to clinical applications.Frontiers in cellular and infection microbiology · 2025Review
- Enhanced detection of RNA modifications and read mapping with high-accuracy nanopore RNA basecalling models.Genome research · 2024Article
- Resolving Sulfation Posttranslational Modifications on a Peptide Hormone using Nanopores.ACS nano · 2024Article
- Understanding Electrophoresis and Electroosmosis in Nanopore Sensing with the Help of the Nanopore Electro-Osmotic Trap.ACS nano · 2024Article
- Nanopores map the acid-base properties of a single site in a single DNA molecule.Nucleic acids research · 2024Article
- Nanopore tweezers show fractional-nucleotide translocation in sequence-dependent pausing by RNA polymerase.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Resolving sulfation PTMs on a plant peptide hormone using nanopore sequencing.bioRxiv : the preprint server for biology · 2024Article
- Detection of phosphorylation post-translational modifications along single peptides with nanopores.Nature biotechnology · 2024Article
- Detection of ribonucleotides embedded in DNA by Nanopore sequencing.Communications biology · 2024Article
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11 authors.
Funding
Abstract
Nanopore DNA sequencing is limited by low base-calling accuracy. Improved base-calling accuracy has so far relied on specialized base-calling algorithms, different nanopores and motor enzymes, or biochemical methods to re-read DNA molecules. Two primary error modes hamper sequencing accuracy: enzyme mis-steps and sequences with indistinguishable signals. We vary the driving voltage from 100 to 200 mV, with a frequency of 200 Hz, across a Mycobacterium smegmatis porin A (MspA) nanopore, thus changing how the DNA strand moves through the nanopore. A DNA helicase moves the DNA through the nanopore in discrete steps, and the variable voltage moves the DNA continuously between these steps. The electronic signal produced with variable voltage is used to overcome the primary error modes in base calling. We found that single-passage de novo base-calling accuracy of 62.7 ± 0.5% with a constant driving voltage improves to 79.3 ± 0.3% with a variable driving voltage. The variable-voltage sequencing mode is complementary to other methods to boost the accuracy of nanopore sequencing and could be incorporated into any enzyme-actuated nanopore sequencing device.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.