ArticleAsian-Australasian journal of animal sciences2020
Characteristics of proteolytic microorganisms and their effects on proteolysis in total mixed ration silages of soybean curd residue.
Article in Asian-Australasian journal of animal sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 28 citations in OpenAlex.
- Role of tannin, saponin, and cumin in inhibiting deamination during ensiling: A molecular docking and molecular dynamics approach.Open veterinary journal · 2025Article
- Effects of cellulase and Lactiplantibacillus plantarum on silage performance, microbial community composition and volatiles of Caragana korshinskii.BMC microbiology · 2025Article
- Effects of irrigation and nitrogen management on phyllosphere microbial communities of silage maize.PeerJ · 2025Article
- Effects of ensiling period and lactic acid bacteria inoculation on aerobic stability of silage in forage infected with leaf spot disease.Frontiers in microbiology · 2025Article
- Isolation, Genomics-Based and Biochemical Characterization of Bacteriocinogenic Bacteria and Their Bacteriocins, Sourced from the Gastrointestinal Tract of Meat-Producing Pigs.International journal of molecular sciences · 2024Article
- Influence of Ensiling Timing and Inoculation on Whole Plant Maize Silage Fermentation and Aerobic Stability (Preliminary Research).Plants (Basel, Switzerland) · 2024Article
- Investigating the efficacy of an exopolysaccharide (EPS)-producing strain Lactiplantibacillus plantarum L75 on oat silage fermentation at different temperatures.Microbial biotechnology · 2024Article
- Characterization of phyllosphere endophytic lactic acid bacteria reveals a potential novel route to enhance silage fermentation quality.Communications biology · 2024Article
- Engineered Biofilm: Innovative Nextgen Strategy for Quality Enhancement of Fermented Foods.Frontiers in nutrition · 2022Review
- Replacing Alfalfa with Paper Mulberry in Total Mixed Ration Silages: Effects on Ensiling Characteristics, Protein Degradation, and In Vitro Digestibility.Animals : an open access journal from MDPI · 2021Article
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5 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
objectiveThe objective of this study was to isolate proteolytic microorganisms and evaluate their effects on proteolysis in total mixed ration (TMR) silages of soybean curd residue.
methodsTMRs were formulated with soybean curd residue, alfalfa or Leymus chinensis hay, corn meal, soybean meal, a vitamin-mineral supplement, and salt in a ratio of 25.0:40.0:30.0:4.0:0.5:0.5, respectively, on a basis of dry matter (DM). The microbial proteinases during ensiling were characterized, the dominate strains associated with proteolysis were identified, and their enzymatic characterization were evaluated in alfalfa (A-TMR) and Leymus chinensis (L-TMR) TMR silages containing soybean curd residue.
resultsBoth A-TMR and L-TMR silages were well preserved, with low pH and high lactic acid concentrations. The aerobic bacteria and yeast counts in both TMR silages decreased to about 105 cfu g-1 FM (Fresh matter) and below the detection limit, respectively. The lactic acid bacteria count increased to 109 cfu g-1 FM. The total microbial proteinases activities reached their maximums during the early ensiling stage and then reduced in both TMR silages with fermentation prolonged. Metalloproteinase was the main proteinase when the total proteinases activities reached their maximums, and when ensiling terminated, metallo and serine proteinases played equally important parts in proteolysis in both TMR silages. Strains in the genera Curtobacterium and Paenibacillus were identified as the most dominant proteolytic bacteria in A-TMR and L-TMR, respectively, and both their proteinases were mainly with metalloproteinase characteristics. In the latter ensiling phase, Enterococcus faecium strains became the major sources of proteolytic enzymes in both TMR silages. Their proteinases were mainly of metallo and serine proteinases classes in this experiment.
conclusionProteolytic aerobic bacteria were substituted by proteolytic lactic acid bacteria during ensiling, and the microbial serine and metallo proteinases in these strains played leading roles in proteolysis in TMR silages.
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