Evidence map›Paper›PMID 31009889›Full record

ArticleJournal of pharmaceutical and biomedical analysis2019

Pharmaceutical and preclinical evaluation of Advax adjuvant as a dose-sparing strategy for ant venom immunotherapy.

Troy Wanandy, Yoshikazu Honda-Okubo, Noel W Davies, Hayley E Rose, Robert J Heddle, Simon G A Brown, Richard J Woodman, Nikolai Petrovsky, Michael D Wiese

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of pharmaceutical and biomedical analysis, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.5field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 8 citations in OpenAlex.

  1. A Pilot Randomised Controlled Dose-Ranging Trial of Ant Venom Immunotherapy With and Without Delta-Inulin Adjuvant.Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 1 country.

Troy WanandyJack Jumper Allergy Program, Royal Hobart Hospital, GPO Box 1061L, Hobart, Tasmania, 7001, Australia; Division of Pharmacy, School of Medicine, University of Tasmania, Private Bag 26, Hobart, Tasmania, 7001, Australia; School of Medicine, University of Tasmania, Private Bag 68, Hobart, Tasmania, 7001, Australia; Department of Pharmacy, Royal Hobart Hospital, GPO Box 1061L, Hobart, Tasmania, 7001, Australia. Electronic address: troy.wanandy@ths.tas.gov.au.
Yoshikazu Honda-OkuboFlinders University, Bedford Park, South Australia, 5042, Australia; Vaxine Pty Ltd, Bedford Park, Adelaide, 5042, Australia.
Noel W DaviesCentral Science Laboratory, University of Tasmania, Private Bag 74, Hobart, Tasmania, 7001, Australia.
Hayley E RoseJack Jumper Allergy Program, Royal Hobart Hospital, GPO Box 1061L, Hobart, Tasmania, 7001, Australia.
Robert J HeddleFlinders University, Bedford Park, South Australia, 5042, Australia; Division of Immunology, SA Pathology, Institute of Medical and Veterinary Science, Frome Road, Adelaide, South Australia, 5000, Australia; Clinical Immunology and Allergy Unit, Royal Adelaide Hospital, North Terrace, Adelaide, South Australia, 5000, Australia; University of Adelaide, Adelaide, South Australia, 5000, Australia.
Simon G A BrownJack Jumper Allergy Program, Royal Hobart Hospital, GPO Box 1061L, Hobart, Tasmania, 7001, Australia; School of Medicine, University of Tasmania, Private Bag 68, Hobart, Tasmania, 7001, Australia; Ambulance Tasmania, Hobart, Tasmania 7000, Australia; Department of Emergency Medicine, Royal Hobart Hospital, GPO Box 1061L, Hobart, Tasmania, 7001, Australia.
Richard J WoodmanFlinders University, Bedford Park, South Australia, 5042, Australia.
Nikolai PetrovskyFlinders University, Bedford Park, South Australia, 5042, Australia; Vaxine Pty Ltd, Bedford Park, Adelaide, 5042, Australia.
Michael D WieseJack Jumper Allergy Program, Royal Hobart Hospital, GPO Box 1061L, Hobart, Tasmania, 7001, Australia; School of Pharmacy and Medical Sciences, University of South Australia, GPO Box 2471, Adelaide, South Australia, 5001, Australia.
Flinders University · AUUniversity of Tasmania · AURoyal Hobart Hospital · AUSouth Australia Pathology · AUUniversity of South Australia · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A major challenge in broader clinical application of Jack Jumper ant venom immunotherapy (JJA VIT) is the scarcity of ant venom which needs to be manually harvested from wild ants. Adjuvants are commonly used for antigen sparing in other vaccines, and thereby could potentially have major benefits to extend JJA supplies if they were to similarly enhance JJA VIT immunogenicity. The purpose of this study was to evaluate the physicochemical and microbiological stability and murine immunogenicity of low-dose JJA VIT formulated with a novel polysaccharide adjuvant referred to as delta inulin or Advax™. Jack Jumper ant venom (JJAV) protein stability was assessed by UPLC-UV, SDS-PAGE, SDS-PAGE immunoblot, and ELISA inhibition. Diffraction light scattering was used to assess particle size distribution of Advax; pH and benzyl alcohol quantification by UPLC-UV were used to assess the physicochemical stability of JJAV diluent, and endotoxin content and preservative efficacy test was used to investigate the microbiological properties of the adjuvanted VIT formulation. To assess the effect of adjuvant on JJA venom immunogenicity, mice were immunised four times with JJAV alone or formulated with Advax adjuvant. JJA VIT formulated with Advax was found to be physicochemically and microbiologically stable for at least 2 days when stored at 4 and 25 °C with a trend for an increase in allergenic potency observed beyond 2 days of storage. Low-dose JJAV formulated with Advax adjuvant induced significantly higher JJAV-specific IgG than a 5-fold higher dose of JJAV alone, consistent with a powerful allergen-sparing effect. The pharmaceutical data provides important guidance on the formulation, storage and use of JJA VIT formulated with Advax adjuvant, with the murine immunogenicity studies providing a strong rationale for a planned clinical trial to test the ability of Advax adjuvant to achieve 4-fold JJAV dose sparing in JJA-allergic human patients.

Indexed as

Adjuvants, ImmunologicAllergensAnimalsAnt VenomsDesensitization, ImmunologicDose-Response Relationship, DrugDrug Evaluation, PreclinicalDrug StabilityFemaleHumansHypersensitivityInulinMiceModels, AnimalAdjuvants, ImmunologicAllergensAnt Venomsdelta inulinInulinAdjuvantsAdvaxAllergyDelta inulinVenom immunotherapy

Identifiers

PMID31009889
PMCPMC7127811
OpenAlexW2938883451

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.